Analysis of an EST library from the dinoflagellate (Symbiodinium sp.) symbiont of reef-building corals

Leggat, W., Hoegh-Guldberg, O., Dove, S. and Yellowlees, D. (2007) Analysis of an EST library from the dinoflagellate (Symbiodinium sp.) symbiont of reef-building corals. Journal of Phycology, 43 5: 1010-1021.


Author Leggat, W.
Hoegh-Guldberg, O.
Dove, S.
Yellowlees, D.
Title Analysis of an EST library from the dinoflagellate (Symbiodinium sp.) symbiont of reef-building corals
Journal name Journal of Phycology   Check publisher's open access policy
ISSN 0022-3646
Publication date 2007-10
Sub-type Article (original research)
DOI 10.1111/j.1529-8817.2007.00387.x
Volume 43
Issue 5
Start page 1010
End page 1021
Total pages 12
Editor Wheeler, P. A.
Place of publication Oxford, England
Publisher Blackwell Publishing
Collection year 2008
Language eng
Subject 270201 Gene Expression
C1
770306 Integrated (ecosystem) assessment and management
Abstract Dinoflagellates (Symbiodinium sp. Freud.) are an obligatory endosymbiont of the reef-building corals. Recent changes to the environment surrounding coral reefs (e.g., global warming) have demonstrated that this endosymbiotic relationship between corals and Symbiodinium is particularly sensitive to environmental changes. Therefore, understanding gene expression patterns of Symbiodinium is critical to understanding why coral reefs are susceptible to global climate change. This study identified 1456 unique expression sequence tags (ESTs) generated for Symbiodinium (clade C3) from the staghorn coral Acropora aspera following exposure to a variety of stresses. Of these, only 10% matched previously reported dinoflagellate ESTs, suggesting that the conditions used in the construction of the library resulted in a novel transcriptome. The function of 561 (44%) of these ESTs could be identified. The majority of these genes coded for proteins involved in posttranslational modification, protein turnover, and chaperones (12.3%); energy production and conversion (12%); or an unknown function (18.6%). The most common transcript found was a homologue to a bacterial protein of unknown function. This algal protein is targeted to the chloroplast and is present in those phototrophs that acquired plastids from the red algal lineage. An additional 48 prokaryote-like proteins were also identified, including the first glycerol-phosphate:phosphate antiporter from dinoflagellates. A protein with similarity to the fungi-archael-bacterial heme catalase peroxidases was also found. A variety of stress genes, in particular heat-shock proteins and proteins involved in ubiquitin cascades, were also identified. This study is the first transcriptome from the unicellular component of a eukaryote-eukaryote symbiosis.
Keyword Plant Sciences
Marine & Freshwater Biology
coral
coral bleaching
dinoflagellate
heat-shock protein
stress
Symbiodinium
ubiquitin
zooxanthellae
Sequence-tag Analysis
Lateral Gene-transfer
Inorganic Carbon
Heat-stress
Ultraviolet-radiation
Elevated-temperature
Molecular Chaperones
Marine Ecology
Genome
Protein
Q-Index Code C1
Q-Index Status Confirmed Code

 
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Created: Sun, 04 May 2008, 22:37:55 EST by Peter Fogarty on behalf of Centre for Marine Studies