Optimized LC–MS/MS quantification method for the detection of piperacillin and application to the development of charged liposaccharides as oral penetration enhancers

Violette, Aude, Faria Cortes, Diego A., Bergeon, Julie A., Falconer, Robert A. and Toth, I. (2008) Optimized LC–MS/MS quantification method for the detection of piperacillin and application to the development of charged liposaccharides as oral penetration enhancers. International Journal of Pharmaceutics, 351 1-2: 152-157. doi:10.1016/j.ijpharm.2007.09.039


Author Violette, Aude
Faria Cortes, Diego A.
Bergeon, Julie A.
Falconer, Robert A.
Toth, I.
Title Optimized LC–MS/MS quantification method for the detection of piperacillin and application to the development of charged liposaccharides as oral penetration enhancers
Journal name International Journal of Pharmaceutics   Check publisher's open access policy
ISSN 0378-5173
Publication date 2008-03-03
Sub-type Article (original research)
DOI 10.1016/j.ijpharm.2007.09.039
Volume 351
Issue 1-2
Start page 152
End page 157
Total pages 5
Place of publication Netherlands
Publisher Elsevier B.V.
Collection year 2008
Language eng
Subject 320307 Medical Biochemistry - Other
C1
780103 Chemical sciences
Abstract Piperacillin, a potent β-lactam antibiotic, is effective in a large variety of Gram+ and Gram− bacterial infections but its administration is limited to the parenteral route as it is not absorbed when given orally. In an attempt to overcome this problem, we have synthesized a novel series of charged liposaccharide complexes of piperacillin comprising a sugar moiety derived from d-glucose conjugated to a lipoamino acid residue with varying side-chain length (cationic entity) and the piperacillin anion. A complete multiple reaction monitoring LC–MS/MS method was developed to detect and characterize the synthesized complexes. The same method was then successfully applied to assess the in vitro apparent permeability values of the charged liposaccharide complexes in Caco-2 monolayers.
Keyword Piperacillin
Charged liposaccharide
Penetration enhancer
LC-MS/MS
Caco-2
Permeability
Q-Index Code C1
Q-Index Status Confirmed Code

 
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Created: Wed, 30 Apr 2008, 13:32:49 EST by Darryl Greensill on behalf of School of Chemistry & Molecular Biosciences