Monocytes immunoselected via the novel monocyte specific molecule, CD300e, differentiate into active migratory dendritic cells

Clark, Georgina J., Jamriska, Lubomira, Rao, Min and Hart, Derek N. J. (2007) Monocytes immunoselected via the novel monocyte specific molecule, CD300e, differentiate into active migratory dendritic cells. Journal of Immunotherapy, 30 3: 303-311. doi:10.1097/01.cji.0000211342.65964.9e


Author Clark, Georgina J.
Jamriska, Lubomira
Rao, Min
Hart, Derek N. J.
Title Monocytes immunoselected via the novel monocyte specific molecule, CD300e, differentiate into active migratory dendritic cells
Journal name Journal of Immunotherapy   Check publisher's open access policy
ISSN 1524-9557
Publication date 2007-01-01
Sub-type Article (original research)
DOI 10.1097/01.cji.0000211342.65964.9e
Volume 30
Issue 3
Start page 303
End page 311
Total pages 9
Editor S. A. Rosenberg
Place of publication United States
Publisher Lippincott Williams & Wilkins
Collection year 2008
Language eng
Subject 320202 Cellular Immunology
C1
730102 Immune system and allergy
Abstract Monocytes, immunoselected using MMRI-1, a monoclonal antibody specific for CD300e, were used to generate dendritic cells (DC). These CD300e immunoselected monocyte-derived DC (MoDC) were compared phenotypically and functionally to CD14 immunoselected MoDC. CD300e and CD14 immunoselected mature MoDC expressed similar levels of the DC marker, CD83 and costimulatory molecules, CD80, CD86, and CD40. Both preparations took up soluble antigen with similar efficiency by pinocytosis and receptor mediated uptake. The CD300e and CD14 immunoselected MoDC also induced comparable CD4+ T lymphocyte allogeneic responses and recall responses to tetanus toxoid. Similar magnitude CD8+ T lymphocyte responses to the naive antigen, MART-1 and the recall antigen, FMP, were induced by both MoDC preparations. Cytokine secretion by each type of MoDC preparation was similar; each secreted interleukin-12, tumor necrosis factor-[alpha], and low levels of interferon-[gamma] but in most cases no interleukin-10. Migration studies confirmed that both types of MoDC migrated towards the chemokine, CCL21 although CD300e immunoselected showed greater migration. Overall, the CD14 immunoselected MoDC had higher spontaneous background migration, compared with the CD300e immunoselected MoDC. Differential signaling from the antibodies used to immunoselect the monocytes may account for the slight differences in migratory capacity. These data identify the CD300e antigen as another monocyte-specific marker that can be used to purify monocytes for differentiation into functionally active MoDC.
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2008 Higher Education Research Data Collection
School of Medicine Publications
 
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Created: Tue, 29 Apr 2008, 22:21:24 EST by Maree Knight on behalf of Faculty Of Health Sciences