Novel Human CD4+ T lymphocyte subpopulations defined by CS300a/c molecule expression

Clark, Georgina J., Rao, Min, Ju, Xinsheng and Hart, Derek N. J. (2007) Novel Human CD4+ T lymphocyte subpopulations defined by CS300a/c molecule expression. Journal of Leukocyte Biology, 82 5: 1126-1135. doi:10.1189/jlb.0107035


Author Clark, Georgina J.
Rao, Min
Ju, Xinsheng
Hart, Derek N. J.
Title Novel Human CD4+ T lymphocyte subpopulations defined by CS300a/c molecule expression
Journal name Journal of Leukocyte Biology   Check publisher's open access policy
ISSN 0741-5400
Publication date 2007
Sub-type Article (original research)
DOI 10.1189/jlb.0107035
Volume 82
Issue 5
Start page 1126
End page 1135
Total pages 10
Editor J. Oppenheim
Place of publication United States
Publisher Federation of American Societies for Experimental Biology
Collection year 2008
Language eng
Subject 320202 Cellular Immunology
320204 Immunogenetics
C1
730102 Immune system and allergy
Formatted abstract
The CD300c (CMRF-35A) and CD300a (CMRF-35H) molecules are leukocyte surface proteins that are part of a larger family of immunoregulatory molecules encoded by a gene complex on human chromosome 17. The CMRF-35 monoclonal antibody binds to an epitope common to both molecules, expressed on most human leukocyte populations, apart from B lymphocytes and a subpopulation of CD4+ and CD8+ T lymphocytes. We describe the CMRF-35pos and CMRF-35– fractions of CD4+ T lymphocytes. The CMRF-35pos fraction can further be divided into CMRF-35++ and CMRF-35+CD4+ T lymphocyte subpopulations. Resting peripheral CD4+ T lymphocytes express CD300a mRNA and very low amounts of CD300c. Activation results in an initial decrease in CD300a gene expression before an increase in both CD300a and CD300c gene expression. The up-regulated expression of these genes was associated with increased CMRF-35 binding to activated T lymphocytes. The CMRF-35– fraction of CD4+ T lymphocytes proliferated to a greater extent than the CMRF-35pos fraction, in response to mitogens or allogeneic antigen. The poor proliferation of the CMRF-35pos CD4+ in response to mitogens was explained by increased apoptosis within this subpopulation. The recall antigen, tetanus toxoid, stimulated the CMRF-35++CD4+CD45RO+ but not the CMRF-35–CD4+CD45RO+ subpopulation. Resting CMRF-35++ CD4+ lymphocytes express low levels of IFN-{gamma} mRNA. Within 18 h following in vitro activation, CMRF-35++ CD4+ lymphocytes express more IFN-{gamma} mRNA and protein compared with the CMRF-35–CD4+ lymphocytes, however, after 24 h, both the CMRF-35+ and CMRF-35–CD4+ T lymphocytes were able to produce IFN-{gamma}. The CMRF-35++CD4+ T lymphocyte population contains the Th1 memory effector cells.
Keyword CD300a
CD300c
T lymphocytes
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2008 Higher Education Research Data Collection
School of Medicine Publications
 
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Created: Tue, 29 Apr 2008, 12:05:03 EST by Maree Knight on behalf of Faculty Of Health Sciences