A PL10 vasa-like gene in the Kuruma shrimp Marsupenaeus japonicus (Bate) is expressed during development in the adult gonad

Sellars, M. J., Lyons, R. E., Grewe, P. M., Vuocolo, T., Leeton, L., Coman, G. J., Degnan, B. M. and Preston, N. P. (2007) A PL10 vasa-like gene in the Kuruma shrimp Marsupenaeus japonicus (Bate) is expressed during development in the adult gonad. Marine Biotechnology, 9 3: 377-387.


Author Sellars, M. J.
Lyons, R. E.
Grewe, P. M.
Vuocolo, T.
Leeton, L.
Coman, G. J.
Degnan, B. M.
Preston, N. P.
Title A PL10 vasa-like gene in the Kuruma shrimp Marsupenaeus japonicus (Bate) is expressed during development in the adult gonad
Formatted title A PL10 vasa-like gene in the Kuruma shrimp, Marsupenaeus japonicus, expressed during development and in adult gonad
Journal name Marine Biotechnology   Check publisher's open access policy
ISSN 1436-2228
Publication date 2007-05
Sub-type Article (original research)
DOI 10.1007/s10126-007-6118-9
Volume 9
Issue 3
Start page 377
End page 387
Total pages 11
Editor J. G. Burgess
S. Miyachi
T. T. Chen
Place of publication United States
Publisher Springer new York LLC
Collection year 2008
Language eng
Subject C1
270208 Molecular Evolution
630303 Aquaculture
Abstract A PL10 vasa-like gene was isolated from the Kuruma shrimp Marsupenaeus japonicus and therefore called Mjpl10. It is differentially expressed during embryonic, larval, and postlarval development, and in female and male gonads. Using absolute real-time reverse transcriptase-polymerase chain reaction (RT-PCR), we demonstrate that Mjpl10 transcripts are present in the two-cell embryo, suggesting it is maternally expressed, and continually at low levels throughout embryogenesis. Mjpl10 expression increases significantly in the first 25 h after hatching (nauplii IV) and then decreases in a linear fashion by 316-fold over the next 52-day period. Its continued expression throughout embryonic and larval development is compatible with a conserved role in early germ cell specification. Transcript levels of Mjpl10 are also detected in the ovary and testes of mature adults.
Keyword Biotechnology & Applied Microbiology
Marine & Freshwater Biology
germ line
Penaeus japonicus
prawn
primordial germ cell
RNA helicase
Primordial Germ-cells
Pacific White Shrimp
Dependent Rna Helicase
Double-stranded-rna
Box Protein Family
Litopenaeus-vannamei
Fenneropenaeus-chinensis
Differential Expression
Molecular-cloning
Crassostrea-gigas
Q-Index Code C1
Q-Index Status Confirmed Code

 
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Created: Thu, 24 Apr 2008, 12:08:23 EST by Sian Rodgie on behalf of School of Biological Sciences