Generalized substitution of isoencoding codons shortens the duration of papillomavirus L1 protein expression in transiently gene-transfected keratinocytes due to cell differentiation

Gu, Wenyi, Ding, Jianmin M., Wang, Xiao, de Kluyver, Rachel L., Saunders, Nicholas A., Frazer, Ian H. and Zhao, Kong-Nan (2007) Generalized substitution of isoencoding codons shortens the duration of papillomavirus L1 protein expression in transiently gene-transfected keratinocytes due to cell differentiation. Nucleic Acids Research, 35 14: 4820-4832. doi:10.1093/nar/gkm496

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
UQ135824.pdf Full text (Open Access) application/pdf 1.51MB 33

Author Gu, Wenyi
Ding, Jianmin M.
Wang, Xiao
de Kluyver, Rachel L.
Saunders, Nicholas A.
Frazer, Ian H.
Zhao, Kong-Nan
Title Generalized substitution of isoencoding codons shortens the duration of papillomavirus L1 protein expression in transiently gene-transfected keratinocytes due to cell differentiation
Journal name Nucleic Acids Research   Check publisher's open access policy
ISSN 0305-1048
1362-4962
Publication date 2007-07-09
Year available 2007
Sub-type Article (original research)
DOI 10.1093/nar/gkm496
Open Access Status DOI
Volume 35
Issue 14
Start page 4820
End page 4832
Total pages 13
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Collection year 2008
Language eng
Abstract Recently we reported that gene codon composition determines differentiation-dependent expression of the PV L1 genes in mouse primary keratinocytes (KCs) in vitro and in vivo (Zhao et al. 2005, Mol. Cell Biol. 25: 8643-8655). Here, we investigated whether generalized substitution of isoencoding codons affects the duration of expression of PV L1 genes in mouse and human KCs in day 1 culture transiently transfected with native (Nat) and codon modified (Mod) L1 genes. Following transient transfection, KC continuously transcribed both Nat and Mod PV L1 genes for at least 12 days, with the levels of L1 mRNAs from the Mod L1 genes significantly higher than those from the Nat L1 genes. However, continuous L1 protein expression at day 9 post-transfection was observed for both mouse and human KCs transfected with the Nat L1 genes only. Further, aa-tRNAs prepared from D8 KC cultures enhanced translation of two PV Nat L1 DNAs in RRL lysate and PV Nat L1 mRNAs in D0 cell-free lysate, whereas aa-tRNAs from D0 KCs enhanced translation of PV Mod L1 mRNAs in D8 cell-free lysate. It appears that aa-tRNAs in less-differentiated and differentiated KCs differentially match the PV Nat and Mod L1 mRNAs to regulate their translations in vitro.
Keyword Keratinocytes
In vitro
In vivo
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 18 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 17 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 21 Apr 2008, 14:41:00 EST by Kylie Hengst on behalf of UQ Diamantina Institute