Assessment and rationalization of genetic diversity of Papua New Guinea taro (Colocasia esculenta) using SSR DNA fingerprinting

Singh, D., Mace, E. S., Godwin, I. D., Mathur, P. N., Okpul, T., Taylor, M., Hunter, D., Kambuou, R., Ramanatha Rao, V. and Jackson, G. (2008) Assessment and rationalization of genetic diversity of Papua New Guinea taro (Colocasia esculenta) using SSR DNA fingerprinting. Genetic Resources and Crop Evolution, 55 6: 811-822. doi:10.1007/s10722-007-9286-6


Author Singh, D.
Mace, E. S.
Godwin, I. D.
Mathur, P. N.
Okpul, T.
Taylor, M.
Hunter, D.
Kambuou, R.
Ramanatha Rao, V.
Jackson, G.
Title Assessment and rationalization of genetic diversity of Papua New Guinea taro (Colocasia esculenta) using SSR DNA fingerprinting
Formatted title
Assessment and rationalization of genetic diversity of Papua New Guinea taro (Colocasia esculenta) using SSR DNA fingerprinting
Journal name Genetic Resources and Crop Evolution   Check publisher's open access policy
ISSN 0925-9864
1573-5109
Publication date 2008-09
Sub-type Article (original research)
DOI 10.1007/s10722-007-9286-6
Volume 55
Issue 6
Start page 811
End page 822
Total pages 12
Place of publication Berlin
Publisher Springer
Collection year 2008
Language eng
Subject 300203 Plant Improvement (Selection, Breeding and Genetic Engineering)
620199 Field crops not elsewhere classified
C1
Abstract Taro (Colocasia esculenta) accessions were collected from 15 provinces of Papua New Guinea (PNG). The collection, totalling 859 accessions was collated for characterization and a core collection of 81 accessions (∼10%) was established on the basis of characterization data generated on 30 agro-morphological descriptors, and DNA fingerprinting using seven SSR primers. The selection of accessions was based on cluster analysis of the morphological data enabling initial selection of 20% accessions. The 20% sample was then reduced and rationalized to 10% based on molecular data generated by SSR primers. This represents the first national core collection of any species established in PNG based on molecular markers. The core has been integrated with core from other Pacific Island countries, contributing to a Pacific regional core collection, which is conserved in vitro in the South Pacific Regional Germplasm Centre at Fiji. The core collection is a valuable resource for food security of the South Pacific region and is currently being utilized by the breeding programmes of small Pacific Island countries to broaden the genetic base of the crop.
Keyword Agro-morphology
Characterization
Colocasia esculenta
Core collection
DNA fingerprinting
Simple sequence repeats (SSR)
Taro
Q-Index Code C1
Q-Index Status Confirmed Code
Additional Notes Published online: 3 November 2007

 
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Created: Fri, 18 Apr 2008, 12:49:23 EST by Kaye Hunt on behalf of School of Land, Crop and Food Sciences