Analysis of Wolbachia protein synthesis in Drosophila in vivo

Sasaki, T., Braig, H. R. and O'Neill, S. L. (1998) Analysis of Wolbachia protein synthesis in Drosophila in vivo. Insect Molecular Biology, 7 2: 101-105. doi:10.1046/j.1365-2583.1998.72057.x

Author Sasaki, T.
Braig, H. R.
O'Neill, S. L.
Title Analysis of Wolbachia protein synthesis in Drosophila in vivo
Journal name Insect Molecular Biology   Check publisher's open access policy
ISSN 0962-1075
Publication date 1998-01-01
Sub-type Article (original research)
DOI 10.1046/j.1365-2583.1998.72057.x
Volume 7
Issue 2
Start page 101
End page 105
Total pages 5
Language eng
Subject 270000 Biological Sciences
270300 Microbiology
270100 Biochemistry and Cell Biology
Abstract Intracellular Wolbachia infections are extremely common in arthropods and exert profound control over the reproductive biology of the host. However, very little is known about the underlying molecular mechanisms which mediate these interactions with the host. We examined protein synthesis by Wolbachia in a Drosophila host in vivo by selective metabolic labelling of prokaryotic proteins and subsequent analysis by 1D and 2D gel electrophoresis. Using this method we could identify the major proteins synthesized by Wolbachia in ovaries and testes of flies. Of these proteins the most abundant was of low molecular weight and showed size variation between Wolbachia strains which correlated with the reproductive phenotype they generated in flies. Using the gel systems we employed it was not possible to identify any proteins of Wolbachia origin in the mature sperm cells of infected flies.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown
Additional Notes To cite this article: T Sasaki, H. R Braig, S. L O'Neill (1998) Analysis of Wolbachia protein synthesis in Drosophila in vivo Insect Molecular Biology 7 (2), 101–105. doi:10.1046/j.1365-2583.1998.72057.x

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
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