wsp Gene sequences from the Wolbachia of filarial nematodes

Bazzocchi, Chiara, Jamnongluk, Wanwisa, O'Neill, Scott L., Anderson, Tim J. C., Genchi, Claudio and Bandi, Claudio (2000) wsp Gene sequences from the Wolbachia of filarial nematodes. Current Microbiology, 41 2: 96-100. doi:10.1007/s002840010100


Author Bazzocchi, Chiara
Jamnongluk, Wanwisa
O'Neill, Scott L.
Anderson, Tim J. C.
Genchi, Claudio
Bandi, Claudio
Title wsp Gene sequences from the Wolbachia of filarial nematodes
Journal name Current Microbiology   Check publisher's open access policy
ISSN 0343-8651
Publication date 2000-08-01
Sub-type Article (original research)
DOI 10.1007/s002840010100
Volume 41
Issue 2
Start page 96
End page 100
Total pages 5
Language eng
Subject 270000 Biological Sciences
270300 Microbiology
270306 Microbial Genetics
270308 Microbial Systematics, Taxonomy and Phylogeny
Abstract Wolbachia endosymbiotic bacteria are widespread in arthropods and are also present in filarial nematodes. Almost all filarial species so far examined have been found to harbor these endosymbionts. The sequences of only three genes have been published for nematode Wolbachia (i.e., the genes coding for the proteins FtsZ and catalase and for 16S rRNA). Here we present the sequences of the genes coding for the Wolbachia surface protein (WSP) from the endosymbionts of eight species of filaria. Complete gene sequences were obtained from the endosymbionts of two different species, Dirofilaria immitis and Brugia malayi. These sequences allowed us to design general primers for amplification of the wsp gene from the Wolbachia of all filarial species examined. For these species, partial WSP sequences (about 600 base pairs) were obtained with these primers. Phylogenetic analysis groups these nematode wsp sequences into a coherent cluster. Within the nematode cluster, wsp-based Wolbachia phylogeny matches a previous phylogeny obtained with ftsZ gene sequences, with a good consistency of the phylogeny of hosts (nematodes) and symbionts (Wolbachia). In addition, different individuals of the same host species (Dirofilaria immitis and Wuchereria bancrofti) show identical wsp gene sequences.
Keyword Wolbachia
endosymbionts
gene sequence
nematodes
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
 
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