The Golgin GCC88 is required for efficient retrograde Transport of Cargo from the early Endosomes to the Trans-Golgi Network

Lieu, Zi Zhao, Derby, Merran C., Teasdale, Rohan D., Hart, Charles, Gunn, Priscilla and Gleeson, Paul A. (2007) The Golgin GCC88 is required for efficient retrograde Transport of Cargo from the early Endosomes to the Trans-Golgi Network. Molecular Biology of the Cell, 18 12: 4979-4991. doi:10.1091/mbc.E07-06-0622

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Author Lieu, Zi Zhao
Derby, Merran C.
Teasdale, Rohan D.
Hart, Charles
Gunn, Priscilla
Gleeson, Paul A.
Title The Golgin GCC88 is required for efficient retrograde Transport of Cargo from the early Endosomes to the Trans-Golgi Network
Journal name Molecular Biology of the Cell   Check publisher's open access policy
ISSN 1059-1524
1939-4586
Publication date 2007-12
Sub-type Article (original research)
DOI 10.1091/mbc.E07-06-0622
Open Access Status File (Publisher version)
Volume 18
Issue 12
Start page 4979
End page 4991
Total pages 12
Editor A. Linstedt
Place of publication USA
Publisher The American Society for Cell Biology
Collection year 2008
Language eng
Subject C1
270103 Protein Targeting and Signal Transduction
780105 Biological sciences
Abstract Retrograde transport pathways from early/recycling endosomes to the trans-Golgi network (TGN) are poorly defined. We have investigated the role of TGN golgins in retrograde trafficking. Of the four TGN golgins, p230/golgin-245, golgin-97, GCC185, and GCC88, we show that GCC88 defines a retrograde transport pathway from early endosomes to the TGN. Depletion of GCC88 in HeLa cells by interference RNA resulted in a block in plasma membrane–TGN recycling of two cargo proteins, TGN38 and a CD8 mannose-6-phosphate receptor cytoplasmic tail fusion protein. In GCC88-depleted cells, cargo recycling was blocked in the early endosome. Depletion of GCC88 dramatically altered the TGN localization of the t-SNARE syntaxin 6, a syntaxin required for endosome to TGN transport. Furthermore, the transport block in GCC88-depleted cells was rescued by syntaxin 6 overexpression. Internalized Shiga toxin was efficiently transported from endosomes to the Golgi of GCC88-depleted cells, indicating that Shiga toxin and TGN38 are internalized by distinct retrograde transport pathways. These findings have identified an essential role for GCC88 in the localization of TGN fusion machinery for transport from early endosomes to the TGN, and they have allowed the identification of a retrograde pathway which differentially selects TGN38 and mannose-6-phosphate receptor from Shiga toxin.
Q-Index Code C1
Q-Index Status Confirmed Code

 
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Created: Fri, 11 Apr 2008, 15:21:51 EST by Cody Mudgway on behalf of Institute for Molecular Bioscience