Analyzing real-time video microscopy: the dyamics and geometry of vescicles and tubules in endocytosis.

Hamilton, N. A., Kerr, M., Burrage, K. and Teasdale, R. D. (2007). Analyzing real-time video microscopy: the dyamics and geometry of vescicles and tubules in endocytosis.. In Bonifacio, J. S., Dasso, M., Harford, J. B., Lippincott-Schwartz, J. and Yamada K. M. (Ed.), Current Protocols in Cell Biology 35 ed. (pp. 4.16.1-1.16.11) USA: John Wiley & Sons.


Author(s) Hamilton, N. A.
Kerr, M.
Burrage, K.
Teasdale, R. D.
Title of chapter Analyzing real-time video microscopy: the dyamics and geometry of vescicles and tubules in endocytosis.
Title of book Current Protocols in Cell Biology
Place of Publication USA
Publisher John Wiley & Sons
Publication year 2007
Edition 35
Editor(s) Bonifacio, J. S.
Dasso, M.
Harford, J. B.
Lippincott-Schwartz, J.
Yamada K. M.
ISBN 0-471-24108-3
Volume number 1
Chapter number 4.16
Start page 4.16.1
End page 1.16.11
Total pages 12
Total chapters 25
Collection year 2008
Subjects B1
239901 Biological Mathematics
780105 Biological sciences
Abstract/Summary With the advent of live cell imaging microscopy, new types of mathematical analyses and measurements are possible. Many of the real-time movies of cellular processes are visually very compelling, but elementary analysis of changes over time of quantities such as surface area and volume often show that there is more to the data than meets the eye. This unit outlines a geometric modeling methodology and applies it to tubulation of vesicles during endocytosis. Using these principles, it has been possible to build better qualitative and quantitative understandings of the systems observed, as well as to make predictions about quantities such as ligand or solute concentration, vesicle pH, and membrane trafficked. The purpose is to outline a methodology for analyzing real-time movies that has led to a greater appreciation of the changes that are occurring during the time frame of the real-time video microscopy and how additional quantitative measurements allow for further hypotheses to be generated and tested.
Keyword(s) endocytosis
video microscopy
endosomal trafficking
membrane trafficking
 
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