Diversity of raft-like domains in late endosomes

Sobo, Komla, Chevallier, Julien, Parton, Robert G., Gruenberg, Jean and van der Goot, F. Gisou (2007) Diversity of raft-like domains in late endosomes. PLoS ONE, 2 4: e391.1-e391.7. doi:10.1371/journal.pone.0000391

Author Sobo, Komla
Chevallier, Julien
Parton, Robert G.
Gruenberg, Jean
van der Goot, F. Gisou
Title Diversity of raft-like domains in late endosomes
Journal name PLoS ONE   Check publisher's open access policy
ISSN 1932-6203
Publication date 2007-04
Sub-type Article (original research)
DOI 10.1371/journal.pone.0000391
Open Access Status DOI
Volume 2
Issue 4
Start page e391.1
End page e391.7
Total pages 7
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Collection year 2008
Language eng
Subject C1
270104 Membrane Biology
780105 Biological sciences
Formatted abstract
Background: Late endosomes, the last sorting station in the endocytic pathway before lysosomes, are pleiomorphic organelles composed of tubular elements as well as vesicular regions with a characteristic multivesicular appearance, which play a crucial role in intracellular trafficking. Here, we have investigated whether, in addition to these morphologically distinguishable regions, late endosomal membranes are additionally sub-compartmentalized into membrane microdomains.

Methodology/Principal Findings: Using sub-organellar fractionation techniques, both with and without detergents, combined with electron microscopy, we found that both the limiting membrane of the organel and the intraluminal vesicles contain raft-type membrane domains. Interestingly, these differentially localized domains vary in protein composition and physico-chemical properties.

Conclusions/Significance: In addition to the multivesicular organization, we find that late endosomes contain cholesterol rich microdomains both on their limiting membrane and their intraluminal vesicles that differ in composition and properties. Implications of these findings for late endosomal functions are discussed.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article number e391

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Created: Fri, 11 Apr 2008, 14:48:26 EST by Jennifer Greder on behalf of Institute for Molecular Bioscience