Determination of Wolbachia genome size by Pulsed-Field Gel Electrophoresis

Sun, L. V., Foster, J. M., Tzertzinis, G., Ono, M., Bandi, C., Slatko, B. and O'Neill, Scott L. (2001) Determination of Wolbachia genome size by Pulsed-Field Gel Electrophoresis. Journal of Bacteriology, 183 7: 2219-2225. doi:10.1128/JB.183.7.2219-2225.2001

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Author Sun, L. V.
Foster, J. M.
Tzertzinis, G.
Ono, M.
Bandi, C.
Slatko, B.
O'Neill, Scott L.
Title Determination of Wolbachia genome size by Pulsed-Field Gel Electrophoresis
Journal name Journal of Bacteriology   Check publisher's open access policy
ISSN 0021-9193
1098-5530
Publication date 2001-04-01
Sub-type Article (original research)
DOI 10.1128/JB.183.7.2219-2225.2001
Open Access Status File (Publisher version)
Volume 183
Issue 7
Start page 2219
End page 2225
Total pages 7
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract Genome sizes of six different Wolbachia strains from insect and nematode hosts have been determined by pulsed-field gel electrophoresis of purified DNA both before and after digestion with rare-cutting restriction endonucleases. Enzymes SmaI, ApaI, AscI, and FseI cleaved the studied Wolbachia strains at a small number of sites and were used for the determination of the genome sizes of wMelPop, wMel, and wMelCS (each 1.36 Mb), wRi (1.66 Mb), wBma (1.1 Mb), and wDim (0.95 Mb). The Wolbachia genomes studied were all much smaller than the genomes of free-living bacteria such as Escherichia coli (4.7 Mb), as is typical for obligate intracellular bacteria. There was considerable genome size variability among Wolbachia strains, especially between the more parasitic A group Wolbachia infections of insects and the mutualistic C and D group infections of nematodes. The studies described here found no evidence for extrachromosomal plasmid DNA in any of the strains examined. They also indicated that the Wolbachia genome is circular.
Keyword genome
Wolbachia
Microbiology
Physical Map
Cytoplasmic Incompatibility
Drosophila-melanogaster
Borrelia-burgdorferi
Genetic-map
Dna
Chromosome
Evolution
Pipientis
Symbiont
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
 
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Created: Thu, 12 Apr 2007, 16:08:51 EST by Ms Roxanna Lane on behalf of School of Biological Sciences