Characterization of Wolbachia host cell range via the in vitro establishment of infections

Dobson, S. L., Marsland, E. J., Veneti, Z., Bourtzis, K. and O'Neill, S. L. (2002) Characterization of Wolbachia host cell range via the in vitro establishment of infections. Applied and Environmental Microbiology, 68 2: 656-660. doi:10.1128/AEM.68.2.656-660.2002

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Author Dobson, S. L.
Marsland, E. J.
Veneti, Z.
Bourtzis, K.
O'Neill, S. L.
Title Characterization of Wolbachia host cell range via the in vitro establishment of infections
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
1098-5336
Publication date 2002-02-01
Sub-type Article (original research)
DOI 10.1128/AEM.68.2.656-660.2002
Open Access Status File (Publisher version)
Volume 68
Issue 2
Start page 656
End page 660
Total pages 5
Place of publication Washington, DC
Publisher American Society for Microbiology
Language eng
Subject 060307 Host-Parasite Interactions
060599 Microbiology not elsewhere classified
Formatted abstract
Maternally transmitted bacteria of the genus Wolbachia are obligate, intracellular symbionts that are frequently found in insects and cause a diverse array of reproductive manipulations, including cytoplasmic incompatibility, male killing, parthenogenesis, and feminization. Despite the existence of a broad range of scientific interest, many aspects of Wolbachia research have been limited to laboratories with insect-rearing facilities. The inability to culture these bacteria outside of the invertebrate host has also led to the existing bias of Wolbachia research toward infections that occur in host insects that are easily reared. Here, we demonstrate that Wolbachia infections can be simply established, stably maintained, and cryogenically stored in vitro using standard tissue culture techniques. We have examined Wolbachia host range by introducing different Wolbachia types into a single tissue culture. The results show that an Aedes albopictus (Diptera: Culicidae) cell line can support five different Wolbachia infection types derived from Drosophila simulans (Diptera: Drosophilidae), Culex pipiens (Culicidae), and Cadra cautella (Lepidoptera: Phycitidae). These bacterial types include infection types that have been assigned to two of the major Wolbachia clades. As an additional examination of Wolbachia host cell range, we demonstrated that a Wolbachia strain from D. simulans could be established in host insect cell lines derived from A. albopictus, Spodoptera frugiperda (Lepidoptera: Noctuidae), and Drosophila melanogaster. These results will facilitate the development of a Wolbachia stock center, permitting novel approaches for the study of Wolbachia infections and encouraging Wolbachia research in additional laboratories.
Keyword Biotechnology & Applied Microbiology
Microbiology
Cytoplasmic Incompatibility
Drosophila-simulans
Phylogenetic Analysis
Nasonia-vitripennis
Culex-pipiens
Populations
Dynamics
Insects
Agent
Line
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Biological Sciences Publications
 
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