Development of an HPLC-MS/MS method for the selective determination of paracetamol metabolites in mouse urine

Hewavitharana, A., Lee, S., Dawson, P. A., Markovich, D. and Shaw, P. N. (2008) Development of an HPLC-MS/MS method for the selective determination of paracetamol metabolites in mouse urine. Analytical Biochemistry, 374 1: 106-111. doi:10.1016/j.ab.2007.11.011


Author Hewavitharana, A.
Lee, S.
Dawson, P. A.
Markovich, D.
Shaw, P. N.
Title Development of an HPLC-MS/MS method for the selective determination of paracetamol metabolites in mouse urine
Journal name Analytical Biochemistry   Check publisher's open access policy
ISSN 0003-2697
Publication date 2008-03-01
Year available 2007
Sub-type Article (original research)
DOI 10.1016/j.ab.2007.11.011
Volume 374
Issue 1
Start page 106
End page 111
Total pages 6
Editor Jakoby, W. B.
Place of publication San Diego, California
Publisher Elsevier BV
Collection year 2008
Language eng
Subject C1
250401 Separation Science
250402 Analytical Spectrometry
780103 Chemical sciences
Abstract An HPLC–MS/MS method has been developed for the selective quantitative analysis of paracetamol and its two major metabolites. The use of tandem MS enabled the detection and quantitation of metabolites in small sample sizes with high sensitivity and selectivity. Isocratic elution using acetonitrile and water containing formic acid combined with electrospray–tandem MS enabled the separation and accurate quantitation of each analyte and the internal standard 3-acetamidophenol. The on-column limits of detection for paracetamol, paracetamol sulfate, and paracetamol glucuronide were 2.4, 1.2, and 1.2 pmol, respectively. The method was applied to quantitate paracetamol and its metabolites in mouse urine. It is highly specific, sensitive, and easily adaptable to measure these analytes in biological fluids of other animals.
Keyword Hplc
Hplc-ms
Apap
Acetaminophen
Metabolites
Tandem Ms
Q-Index Code C1
Q-Index Status Confirmed Code
Additional Notes Available online November 2007

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2008 Higher Education Research Data Collection
School of Biomedical Sciences Publications
 
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Created: Tue, 01 Apr 2008, 10:08:21 EST by Elizabeth Pyke on behalf of School of Pharmacy