Adipose differentiation of bone marrow-derived mesenchymal stem cells using Pluronic F-127 hydrogel in vitro

Vashi, A. V., Keramidaris, E., Abberton, K. M., Morrison, W. A., Wilson, J. L., O'Connor, A. J., Cooper-White, J. J. and Thompson, E. W. (2008) Adipose differentiation of bone marrow-derived mesenchymal stem cells using Pluronic F-127 hydrogel in vitro. Biomaterials, 29 5: 573-579.


Author Vashi, A. V.
Keramidaris, E.
Abberton, K. M.
Morrison, W. A.
Wilson, J. L.
O'Connor, A. J.
Cooper-White, J. J.
Thompson, E. W.
Title Adipose differentiation of bone marrow-derived mesenchymal stem cells using Pluronic F-127 hydrogel in vitro
Formatted title
Journal name Biomaterials   Check publisher's open access policy
ISSN 0142-9612
Publication date 2008-02
Year available 2007
Sub-type Article (original research)
DOI 10.1016/j.biomaterials.2007.10.017
Volume 29
Issue 5
Start page 573
End page 579
Total pages 6
Editor D. F. Williams
Place of publication Netherlands
Publisher Elsevier BV
Collection year 2008
Language eng
Subject 291503 Biomaterials
780105 Biological sciences
C1
Abstract Due to increasing clinical demand for adipose tissue, a suitable scaffold for engineering adipose tissue constructs is needed. In this study, we have developed a three-dimensional (3-D) culture system using bone marrow-derived mesenchymal stem cells (BM-MSC) and a Pluronic F-127 hydrogel scaffold as a step towards the in vitro tissue engineering of fat. BM-MSC were dispersed into a Pluronic F-127 hydrogel with or without type I collagen added. The adipogenic differentiation of the BM-MSC was assessed by cellular morphology and further confirmed by Oil Red O staining. The BM-MSC differentiated into adipocytes in Pluronic F-127 in the presence of adipogenic stimuli over a period of 2 weeks, with some differentiation present even in absence of such stimuli. The addition of type I collagen to the Pluronic F-127 caused the BM-MSC to aggregate into clumps, thereby generating an uneven adipogenic response, which was not desirable.
Keyword Adipose tissue engineering
Thermally responsive material
Copolymer
Cell culture
Lipid
In vitro test
Q-Index Code C1
Q-Index Status Provisional Code

 
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Created: Mon, 31 Mar 2008, 15:47:09 EST by Gail Smith on behalf of School of Chemical Engineering