Interaction of metal salts with cytoskeletal motor protein systems

Thier, R., Bonacker, D., Stoiber, T., Bohm, K. J., Wang, M., Unger, E., Bolt, H. M. and Degen, G. (2003) Interaction of metal salts with cytoskeletal motor protein systems. Toxicology Letters, 140 75-81. doi:10.1016/S0378-4274(02)00502-7

Author Thier, R.
Bonacker, D.
Stoiber, T.
Bohm, K. J.
Wang, M.
Unger, E.
Bolt, H. M.
Degen, G.
Title Interaction of metal salts with cytoskeletal motor protein systems
Journal name Toxicology Letters   Check publisher's open access policy
ISSN 0378-4274
Publication date 2003
Sub-type Article (original research)
DOI 10.1016/S0378-4274(02)00502-7
Volume 140
Start page 75
End page 81
Total pages 7
Place of publication Clare
Publisher Elsevier Sci Ireland Ltd
Language eng
Abstract Interactions of chemicals with the microtubular network of cells may lead to genotoxicity. Micronuclei (MN) might be caused by interaction of metals with tubulin and/or kinesin. The genotoxic effects of inorganic lead and mercury salts were studied using the MN assay and the CREST analysis in V79 Chinese hamster fibroblasts. Effects on the functional activity of motor protein systems were examined by measurement of tubulin assembly and kinesin-driven motility. Lead and mercury salts induced MN dose-dependently. The no-effect-concentration for NIN induction was 1.1 muM PbCl2, 0.05 muM Pb(OAc)(2) and 0.01 muM HgCl2. The in vitro results obtained for PbCl2 correspond to reported MN induction in workers occupationally exposed to lead, starting at 1.2 muM Hg(II) (Vaglenov et al., 2001, Environ. Health Perspect. 109, 295-298). The CREST Analysis indicate aneugenic effects of Pb(II) and aneugenic and additionally clastogenic effects of Hg(II). Lead (chloride, acetate, and nitrate) and mercury (chloride and nitrate) interfered dose-dependently with tubulin assembly in vitro. The no-effect-concentration for lead salts in this assay was 10 muM. Inhibition of tubulin assembly by mercury started at 2 muM The gliding velocity of microtubules along immobilised kinesin molecules was affected by 25 muM Pb(NO3)(2) and 0.1 muM HgCl2 in a dose-dependent manner. Our data support the hypothesis that lead and mercury genotoxicity may result, at least in part, via disturbance of chromosome segregation via interaction with cytoskeletal proteins. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.
Keyword Toxicology
micronucleus test
cytoskeletal motor protein
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Biomedical Sciences Publications
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Created: Fri, 28 Mar 2008, 15:21:02 EST