Biological monitoring in workers in a nitrobenzene reduction plant: haemoglobin versus serum albumin adducts

Thier, R, Lewalter, J, Selinski, S and Bolt, HM (2001) Biological monitoring in workers in a nitrobenzene reduction plant: haemoglobin versus serum albumin adducts. International Archives of Occupational And Environmental Health, 74 7: 483-488.


Author Thier, R
Lewalter, J
Selinski, S
Bolt, HM
Title Biological monitoring in workers in a nitrobenzene reduction plant: haemoglobin versus serum albumin adducts
Journal name International Archives of Occupational And Environmental Health   Check publisher's open access policy
ISSN 0340-0131
Publication date 2001
Sub-type Article (original research)
DOI 10.1007/s004200100250
Volume 74
Issue 7
Start page 483
End page 488
Total pages 6
Place of publication New York
Publisher Springer-verlag
Language eng
Abstract The high priority of monitoring workers exposed to nitrobenzene is a consequence of clear findings of experimental carcinogenicity of nitrobenzene and the associated evaluations by the International Agency for Research on Cancer. Eighty male employees of a nitrobenzene reduction plant, with potential skin contact with nitrobenzene and aniline, participated in a current medical surveillance programme. Blood samples were routinely taken and analysed for aniline, 4-aminodiphenyl (4-ADP) and benzidine adducts of haemoglobin (Hb) and human serum albumin (HSA). Also, levels of methaemoglobin (Met-Hb) and of carbon monoxide haemoglobin (CO-Hb) were monitored. Effects of smoking were straightforward. Using the rank sum test of Wilcoxon, we found that very clear-cut and statistically significant smoking effects (about 3-fold increases) were apparent on CO-Hb (P = 0.00085) and on the Hb adduct of 4-ADP (P = 0.0006). The mean 'aniline-Hb adduct level in smokers was 1.5 times higher than in non-smokers; the significance (P = 0.05375) was close to the 5% level. The strongest correlation was evident between the Hb and HSA adducts of aniline (r(s) = 0.846). Less pronounced correlations (but with P values < 0.02) appeared between aniline-Hb and 4-ADP-Hb adducts (r(s) = 0.388), between 4-ADP and 4-ADP-HSA adducts (r(s) = 0.373), and between 4-ADP-Hb and aniline-HSA adducts (r(s) = 0.275). In view of the proposal for additional use of the aniline-HSA adduct for biological monitoring, particularly in cases of acute overexposures or poisonings, the strong correlation of the Hb and HSA conjugates is noteworthy; the ratio aniline-HSA:aniline-Hb was 1:42 for the entire cohort.
Keyword Public, Environmental & Occupational Health
nitrobenzene
aniline
biological monitoring
haemoglobin adducts
serum albumin adducts
Hemoglobin Adducts
Aromatic-amines
Acrylonitrile
Metabolism
Toxicity
Exposure
Aniline
Rats
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
 
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