Production of polyclonal antisera

Cooper, Helen M. and Paterson, Yvonne (2008). Production of polyclonal antisera. In Frederick M. Ausubel, Roger Brent, Robert E. Kingston, David D. Moore, J. G. Seidman and Kevin Struhl (Ed.), Current protocols in molecular biology (pp. 11.12.1-11.12.10) New York, U.S.: John Wiley. doi:10.1002/0471142727.mb1112s81


Author Cooper, Helen M.
Paterson, Yvonne
Title of chapter Production of polyclonal antisera
Title of book Current protocols in molecular biology
Place of Publication New York, U.S.
Publisher John Wiley
Publication Year 2008
Sub-type Research book chapter (original research)
DOI 10.1002/0471142727.mb1112s81
Open Access Status
ISBN 9780471142720
ISSN 1934-3639
1934-3647
Editor Frederick M. Ausubel
Roger Brent
Robert E. Kingston
David D. Moore
J. G. Seidman
Kevin Struhl
Chapter number 11
Start page 11.12.1
End page 11.12.10
Total pages 10
Collection year 2009
Language eng
Subjects 06 Biological Sciences
11 Medical and Health Sciences
0601 Biochemistry and Cell Biology
1101 Medical Biochemistry and Metabolomics
1107 Immunology
C1
Abstract/Summary Much of modern biology and biochemistry relies on the availability of highly specific antibodies for use in such ubiquitous techniques as immunohistochemistry, ELISAs, immunoprecipitation, and immunoblotting. Thus, the generation of large quantities of specific antibodies directed against proteins or peptides of interest is essential to the success of both basic and applied research programs. In addition, with the advent of antibody-based proteomic strategies for profiling protein expression and post-translational modification, a requirement for timely production of specific antibodies has been exemplified. Polyclonal antibodies derived from animals immunized with purified proteins or peptides are particularly valuable for use in the laboratory. This unit provides protocols for the production of polyclonal antisera specific for protein antigens using rabbits, rats, mice, and hamsters
Keyword Polyclonal antiserum
Adjuvant
Antigen
Immunization
Q-Index Code C1
Q-Index Status Confirmed Code

 
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Created: Wed, 19 Mar 2008, 09:44:00 EST