Molecular diagnosis of medical viruses.

Ratcliff, R. M., Chang, G., TuckWeng, K. and Sloots, T. P. (2007) Molecular diagnosis of medical viruses.. Current Issues in Molecular Biology, 9 87-102.

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Author Ratcliff, R. M.
Chang, G.
TuckWeng, K.
Sloots, T. P.
Title Molecular diagnosis of medical viruses.
Journal name Current Issues in Molecular Biology   Check publisher's open access policy
ISSN 1467-3037
Publication date 2007
Sub-type Critical review of research, literature review, critical commentary
Open Access Status File (Publisher version)
Volume 9
Start page 87
End page 102
Total pages 16
Editor A. K. Bej
A. Cabibbo
T. R. Callaway
R. H. Datar
J. Giovannetti
L. Good
R. Grant
J. D. McDonald
K. M. Peterson
P. Rangel
I. Rowland
G. Symonds
Place of publication United Kingdom
Publisher Horizon Scientific Press
Collection year 2008
Language eng
Subject C1
730204 Child health
730101 Infectious diseases
270303 Virology
270304 Infectious Agents
Abstract The diagnosis of infectious diseases has been revolutionized by the development of molecular techniques, foremost with the applications of the polymerase chain reaction (PCR). The achievable high sensitivity and ease with which the method can be used to detect any known genetic sequence have led to its wide application in the life sciences. More recently, real-time PCR assays have provided additional major contributions, with the inclusion of an additional fluorescent probe detection system resulting in an increase in sensitivity over conventional PCR, the ability to confirm the amplification product and to quantitate the target concentration. Further, nucleotide sequence analysis of the amplification products has facilitated epidemiological studies of infectious disease outbreaks, and the monitoring of treatment outcomes for infections, in particular with viruses which mutate at high frequency. This review discusses the applications of qualitative and quantitative real-time PCR, nested PCR, multiplex PCR, nucleotide sequence analysis of amplified products and quality assurance with nucleic acid testing (NAT) in diagnostic laboratories.
Keyword real time PCR assays
fluorescent probe detection system
nucleotide sequence analysis of amplified products
Q-Index Code C1
Q-Index Status Confirmed Code
Additional Notes This document is a journal review.

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Excellence in Research Australia (ERA) - Collection
2008 Higher Education Research Data Collection
Clinical Medical Virology Centre Publications
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Citation counts: TR Web of Science Citation Count  Cited 69 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 18 Mar 2008, 11:49:29 EST by Lesley Arnicar on behalf of Clinical Medical Virology Centre