JHE in Gryllus assimilis: Cloning, sequence-activity associations and phylogeny

Crone, E. J., Zera, A. J., Anand, A., Oakeshott, J. G., Sutherland, T. D., Russell, R. J., Harshman, L. G., Hoffmann, F. G. and Claudianos, C. (2007) JHE in Gryllus assimilis: Cloning, sequence-activity associations and phylogeny. Insect Biochemistry and Molecular Biology, 37 12: 1359-1365. doi:10.1016/j.ibmb.2007.08.005


Author Crone, E. J.
Zera, A. J.
Anand, A.
Oakeshott, J. G.
Sutherland, T. D.
Russell, R. J.
Harshman, L. G.
Hoffmann, F. G.
Claudianos, C.
Title JHE in Gryllus assimilis: Cloning, sequence-activity associations and phylogeny
Formatted title
JHE in Gryllus assimilis: Cloning, sequence-activity associations and phylogeny
Journal name Insect Biochemistry and Molecular Biology   Check publisher's open access policy
ISSN 0965-1748
1879-0240
Publication date 2007-12-01
Sub-type Article (original research)
DOI 10.1016/j.ibmb.2007.08.005
Volume 37
Issue 12
Start page 1359
End page 1365
Total pages 7
Editor A.S. Raikhel
R. Feyereisen
Place of publication London, United Kingdom
Publisher Pergamon-Elsevier Science
Collection year 2008
Language eng
Subject C1
270205 Genetic Development (incl. Sex Determination)
730210 Environmental health
Formatted abstract
The 458 amino acid sequence of a mature JHE protein from the cricket Gryllus assimilis was identified after isolating the partial cDNA sequence encoding this protein from a fat body and midgut cDNA library. This hemimetabolan JHE sequence shows over 40% amino acid similarity to the known JHE sequences of several holometabolous insects. It also includes previously determined peptide sequences for G. assimilis JHE as well as two other motifs associated with JHE enzymes in holometabolous insects. The predicted molecular weight of the protein agrees with that of the JHE previously purified from G. assimilis. Partial genomic sequence encoding the Jhe contains two large (1330 and 2918 bp) introns. No coding DNA sequence variation was observed over a 1293 bp region between selected lines differing six to eight-fold in hemolymph JHE activity. However, a 19 bp indel was found in one of the introns; the insertion was strongly associated with elevated hemolymph activity, both in the selected lines and in the F-2 progeny of crosses between them. Phylogenetic analyses localised the G. assimilis JHE to a clade containing dipteran and coleopteran JHEs, with lepidopteran JHEs occurring in a separate clade.
Keyword Biochemistry & Molecular Biology
Entomology
Juvenile hormone esterase gene
Gryllus assimilis
Hemimetabolous insect
Indel polymorphism
Phylogeny
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 15 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 18 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 19 Feb 2008, 01:50:03 EST