Diffusion tensor magnetic resonance imaging and tract-tracing analysis of probst bundle structure in Netrin1- and DCC-deficient mice

Ren, T, Zhang, JY, Plachez, C, Mori, S and Richards, LJ (2007) Diffusion tensor magnetic resonance imaging and tract-tracing analysis of probst bundle structure in Netrin1- and DCC-deficient mice. Journal of Neuroscience, 27 39: 10345-10349. doi:10.1523/JNEUROSCI.2787-07.2007

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Author Ren, T
Zhang, JY
Plachez, C
Mori, S
Richards, LJ
Title Diffusion tensor magnetic resonance imaging and tract-tracing analysis of probst bundle structure in Netrin1- and DCC-deficient mice
Journal name Journal of Neuroscience   Check publisher's open access policy
ISSN 0270-6474
Publication date 2007
Sub-type Article (original research)
DOI 10.1523/JNEUROSCI.2787-07.2007
Open Access Status File (Publisher version)
Volume 27
Issue 39
Start page 10345
End page 10349
Total pages 5
Editor Van Essen, DC
Place of publication Washington
Publisher Soc Neuroscience
Collection year 2008
Language eng
Subject C1
270210 Neurogenetics
730104 Nervous system and disorders
Abstract In many cases of callosal dysgenesis in both human patients and mouse models, misguided fibers from the cortex form abnormal bilateral, barrel-shaped structures known as Probst bundles. Because little is known about how axons are arranged within these anomalous fiber bundles, understanding this arrangement may provide structural and molecular insights into how axons behave when they are misguided in vivo. Previous studies described these bundles as longitudinal swirls of axons that fail to cross the midline (Ozaki et al., 1987). However, recent studies on human acallosal patients using diffusion tensor magnetic resonance imaging (DTMRI) technology suggest that axons project in an anteroposterior direction within the Probst bundle ( Lee et al., 2004; Tovar-Moll et al., 2007). This led us to ask the question, is DTMRI an accurate method for analyzing axonal tracts in regions of high axon overlap and disorganization, or is our current perception of axon arrangement within these bundles inaccurate? Using DTMRI, immunohistochemistry, and carbocyanine dye tract-tracing studies, we analyzed the Probst bundles in both Netrin1 and deleted in colorectal cancer (DCC) mutant mice. Our findings indicate that DTMRI can accurately demonstrate fiber tract orientation and morphology where axons are in ordered arrays such as in the dorsal part of the bundle. In ventral areas, where the axons are disorganized, no coordinated diffusion is apparent via DTMRI. In these regions, a higher-resolution approach such as tract tracing is required. We conclude that in DCC and Netrin1 mutant mice, guidance mechanisms remain in the dorsal part of the tract but are lost ventrally.
Keyword Neurosciences
corpus callosum
axon guidance
brain development
agenesis of the corpus callosum (ACC)
cerebral cortex
magnetic resonance imaging
Colorectal-cancer Dcc
Corpus-callosum
Netrin-1
Projections
Dysgenesis
Guidance
Brain
Q-Index Code C1
Q-Index Status Confirmed Code

 
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Created: Mon, 18 Feb 2008, 15:11:56 EST