Distinct impact of alcoholism, smoking and co-abuse on gene expression in the human nucleus accumbens

Flatscher-Bader, T., Matsumoto, I. and Wilce, P. A. (2007). Distinct impact of alcoholism, smoking and co-abuse on gene expression in the human nucleus accumbens. In: 21st Biennial Meeting of the International Society for Neurochemistry and the 38th Annual Meeting of the American Society for Neurochemistry: Abstracts. 21st Biennial Meeting of the International Society for Neurochemistry; 38th Annual Meeting of the American Society for Neurochemistry, Cancún, Mexico, (37-38). 19-24 August 2007. doi:10.1111/j.0022-3042.2007.04726.x


Author Flatscher-Bader, T.
Matsumoto, I.
Wilce, P. A.
Title of paper Distinct impact of alcoholism, smoking and co-abuse on gene expression in the human nucleus accumbens
Conference name 21st Biennial Meeting of the International Society for Neurochemistry; 38th Annual Meeting of the American Society for Neurochemistry
Conference location Cancún, Mexico
Conference dates 19-24 August 2007
Proceedings title 21st Biennial Meeting of the International Society for Neurochemistry and the 38th Annual Meeting of the American Society for Neurochemistry: Abstracts   Check publisher's open access policy
Journal name Journal of Neurochemistry   Check publisher's open access policy
Place of Publication Oxford, U.K.
Publisher Wiley-Blackwell Publishing
Publication Year 2007
Sub-type Published abstract
DOI 10.1111/j.0022-3042.2007.04726.x
ISSN 0022-3042
1471-4159
Volume 102
Issue Supp. 1
Start page 37
End page 38
Total pages 2
Language eng
Formatted Abstract/Summary
The incidence of alcohol and tobacco co-abuse is 80–90% but the molecular mechanism underlying this comorbidity is virtually unknown. However, interactions between the drugs have important implications for the development of, and recovery from, drug dependence. To investigate the effects of smoking and alcoholism and the interaction of the two behaviours on gene expression in the human nucleus accumbens (NA), the expression profile of 20 alcoholic and control cases, with and without smoking comorbidity, was determined using cDNA microarrays. Changes to expression were revealed by ANOVA and hierarchical gene clusters. DAVID software (http://david.abcc.ncifcrf.gov/) was used for gene annotation, mapping to canonical pathways and functional grouping based on kappa statistics. Hierarchical clustering of smokingsensitive genes grouped smoking cases and predicted smoking comorbidity in alcoholics. Alcohol-sensitive genes were less dominant and did not distinguish alcoholism in chronic smokers. Smoking-sensitive genes were concerned with the regulation of transcription, RNA metabolism and mitogen-activated protein kinase pathway. Alcohol-sensitive genes were associated with protein and RNA turnover and stressresponses. One small but prominent alcohol-sensitive gene set encoding proteins involved in regulation of the actin cytoskeleton and focal adhesion. The two drugs interacted to influence expression of gene clusters involved in signalling and brain-specific functions such as morphogenesis, axonogenesis or nerve ensheathment. We conclude that comorbidity may profoundly impact on plasticity within the NA and particularly alter structural connectivity and further, that smoking plays a dominant role in inducing these changes.
Subjects 320307 Medical Biochemistry - Other
270107 Cell Neurochemistry
CX
730104 Nervous system and disorders
730205 Substance abuse
Keyword Biochemistry & Molecular Biology
Neurosciences
Q-Index Code EX
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Special Issue: "This supplement also represents Volume 38 of the Transactions of the American Society for Neurochemistry". Published under "Synaptic plasticity and neurodegeneration" as Abstract P.32.

 
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Created: Tue, 19 Feb 2008, 00:50:02 EST