Diversity of toxic components from the venom of the evolutionarily distinct black whip snake, Demansia vestigiata

St Pierre, L., Birrell, G. W., Earl, S. T., Wallis, T. P., Gorman, J. J., de Jersey, J., Masci, P. P. and Lavin, M. F. (2007) Diversity of toxic components from the venom of the evolutionarily distinct black whip snake, Demansia vestigiata. Journal of Proteome Research, 6 8: 3093-3107. doi:10.1021/pr0701613


Author St Pierre, L.
Birrell, G. W.
Earl, S. T.
Wallis, T. P.
Gorman, J. J.
de Jersey, J.
Masci, P. P.
Lavin, M. F.
Title Diversity of toxic components from the venom of the evolutionarily distinct black whip snake, Demansia vestigiata
Formatted title
Diversity of toxic components from the venom of the evolutionarily distinct black whip snake, Demansia vestigiata
Journal name Journal of Proteome Research   Check publisher's open access policy
ISSN 1535-3893
Publication date 2007
Sub-type Article (original research)
DOI 10.1021/pr0701613
Volume 6
Issue 8
Start page 3093
End page 3107
Total pages 15
Place of publication Washington
Publisher American Chemical Society
Collection year 2008
Language eng
Subject 270100 Biochemistry and Cell Biology
110201 Cardiology (incl. Cardiovascular Diseases)
110106 Medical Biochemistry: Proteins and Peptides (incl. Medical Proteomics)
Formatted abstract
Included among the more than 300 species of elapid snakes worldwide is the Australian genus Demansia, or whip snakes. Despite evidence to suggest adverse clinical outcomes from envenomation by these snakes, together with confusion on their true phylogenetic relationship to other Australian elapids, not a single toxin sequence has previously been reported from the venom of a Demansia species. We describe here a combined proteomic and transcriptomic approach characterizing the venom from the black whip snake, Demansia vestigiata. A total of 13 distinct toxin families were identified, including homologues of all of the major toxic components previously reported from the venom of other Australian elapids, such as factor X-like prothrombin activators, neurotoxins, phospholipases, cysteine rich secretory proteins, textilinin-like molecules, nerve growth factors, L-amino acid oxidases, vespryns, 5' nucleotidases, metalloproteinases, and C-type lectins as well as a novel dipeptidyl peptidase family. Phylogenetic analysis of these sequences revealed an early evolutionary split of the black whip snake from all other characterized Australian snakes, with a low degree of sequence identity between D. vestigiata and the other snakes, across all toxin families. The results of this study have important implications not only for the further characterization of venom from whip snakes, but also for our understanding of the evolutionary relationship of Australian snake species.
Keyword Biochemical Research Methods
Demansia Vestigiata
Black Whip Snake
Toxin
Venom
Proteomics
Cdna Cloning
Snake Venomics
Potent Presynaptic Neurotoxin
Gated Ion Channels
King Cobra Venom
Pseudonaja-textilis
Phospholipase A(2)
Pharmacological-properties
Prothrombin Activators
Australian Elapids
Mass-spectrometry
Peptidase-iv
Q-Index Code C1

 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 15 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 18 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 18 Feb 2008, 14:39:34 EST