Crystallization and preliminary X-ray diffraction of the Munc18c-syntaxin4(1-29) complex

Latham, C. F., Hu, S. H., Gee, C. L., Armishaw, C. J., Alewood, P. F., James, D. E. and Martin, J. L. (2007) Crystallization and preliminary X-ray diffraction of the Munc18c-syntaxin4(1-29) complex. Acta Crystallographica. Section F: Structural Biology and Crystallization Communications, 63 pt 6: 524-528. doi:10.1107/S1744309107022361

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Author Latham, C. F.
Hu, S. H.
Gee, C. L.
Armishaw, C. J.
Alewood, P. F.
James, D. E.
Martin, J. L.
Title Crystallization and preliminary X-ray diffraction of the Munc18c-syntaxin4(1-29) complex
Formatted title
Crystallization and preliminary X-ray diffraction of the Munc18c-syntaxin41-29 complex
Journal name Acta Crystallographica. Section F: Structural Biology and Crystallization Communications   Check publisher's open access policy
ISSN 1744-3091
Publication date 2007
Sub-type Article (original research)
DOI 10.1107/S1744309107022361
Open Access Status File (Publisher version)
Volume 63
Issue pt 6
Start page 524
End page 528
Total pages 5
Place of publication Malden, MA, United States
Publisher Wiley-Blackwell
Collection year 2008
Language eng
Subject C1
250302 Biological and Medical Chemistry
780105 Biological sciences
780103 Chemical sciences
Abstract The production of diffraction-quality crystals of Munc18c, a protein involved in regulating vesicular exocytosis in mammals, is reported. The diffraction resolution of Munc18c crystals was optimized by (i) cocrystallizing with a peptide fragment of the Munc18c functional binding partner syntaxin4, (ii) using nanolitre free- interface diffusion crystallization- screening chips and microlitre hanging- drop vapour diffusion and (iii) applying a post- crystallization dehydration treatment. Crystals belonging to the cubic space group P2(1)3, with unit-cell parameters a = b = c = 170.8 angstrom, alpha = beta = gamma = 90 degrees, were generated that diffract to 3.7 angstrom resolution on a laboratory X- ray source.
Keyword Biochemical Research Methods
Biochemistry & Molecular Biology
Biophysics
Crystallography
Protein Crystals
Trafficking
Munc18c
Q-Index Code C1
Q-Index Status Confirmed Code

 
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Created: Mon, 18 Feb 2008, 14:23:05 EST