A model of experimental autoimmune encephalomyelitis (EAE) in C57BL/6 mice for the characterisation of intervention therapies

Peiris, Madusha, Monteith, Gregory R, Roberts-Thomson, Sarah J. and Cabot, Peter J. (2007) A model of experimental autoimmune encephalomyelitis (EAE) in C57BL/6 mice for the characterisation of intervention therapies. Journal of Neuroscience Methods, 163 2: 245-254. doi:10.1016/j.jneumeth.2007.03.013


Author Peiris, Madusha
Monteith, Gregory R
Roberts-Thomson, Sarah J.
Cabot, Peter J.
Title A model of experimental autoimmune encephalomyelitis (EAE) in C57BL/6 mice for the characterisation of intervention therapies
Journal name Journal of Neuroscience Methods   Check publisher's open access policy
ISSN 0165-0270
Publication date 2007-07-30
Sub-type Article (original research)
DOI 10.1016/j.jneumeth.2007.03.013
Volume 163
Issue 2
Start page 245
End page 254
Total pages 10
Editor G. A. Gerhardt
V. Crunelli
Place of publication Amsterdam, The Netherlands
Publisher Elsevier Science BV
Collection year 2008
Language eng
Subject C1
320501 Pharmaceutical Sciences and Pharmacy
730104 Nervous system and disorders
Abstract Multiple sclerosis (MS) and its different forms are Studied in the animal model experimental autoimmune encephalomyelitis (EAE). Relapsing-remitting MS. the most common form of the disease can be induced in mice where clinical symptoms fluctuate in severity over time. However. the animal model does not experience periods of recovery where clinical signs are absent, unlike the human disease. We have developed a novel model of relapsing-remitting EAE in C57BL/6 mice immunised with myelin oligodendrocyte glycoprotein (MOG) peptide and Quil A as adjuvant. These animals have relapses that are followed by periods of recovery, during which time the animals do not exhibit illness. Furthermore. administration of the PPAR gamma agonist pioglitazone prior to a predicted relapse prevents the expected development of symptoms in a dose-dependent fashion. Immune cell infiltration into white matter of the CNS and decreased production of inflammatory cytokine IFN-gamma in treated animals were also observed. Our model will be a valuable tool in assessing intervention therapies for RR-MS sufferers. (c) 2007 Elsevier B.V. All rights reserved.
Keyword Biochemical Research Methods
Neurosciences
EAE
multiple sclerosis
model
inflammation
Myelin Oligodendrocyte Glycoprotein
Proliferator-activated Receptors
Mog-induced Eae
Multiple-sclerosis
Ppar-gamma
Il-6-deficient Mice
Freund Adjuvant
Nervous-system
Animal-models
T-cell
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2008 Higher Education Research Data Collection
School of Pharmacy Publications
 
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Created: Mon, 18 Feb 2008, 14:21:01 EST