Using the neurosphere assay to quantify neural stem cells in vivo

Marshall, G. P., Reynolds, B. A. and Laywell, E. D. (2007) Using the neurosphere assay to quantify neural stem cells in vivo. Current Pharmaceutical Biotechnology, 8 3: 141-145. doi:10.2174/138920107780906559

Author Marshall, G. P.
Reynolds, B. A.
Laywell, E. D.
Title Using the neurosphere assay to quantify neural stem cells in vivo
Journal name Current Pharmaceutical Biotechnology   Check publisher's open access policy
ISSN 1389-2010
Publication date 2007-06
Sub-type Critical review of research, literature review, critical commentary
DOI 10.2174/138920107780906559
Volume 8
Issue 3
Start page 141
End page 145
Total pages 5
Place of publication Sharjah
Publisher Bentham Science Publ Ltd
Language eng
Subject 1115 Pharmacology and Pharmaceutical Sciences
1004 Medical Biotechnology
Abstract Since their initial description in 1992, neurospheres have appeared in some aspect of more than a thousand published studies. Despite their ubiquitous presence in the scientific literature, there is little consensus regarding the fundamental defining characteristics of neurospheres; thus, there is little agreement about what, if anything, the neurosphere assay can tell us about the relative abundance or behavior of neural stem cells in vivo. In this review we will examine some of the common features of neurospheres, and ask if these features should be interpreted as a proxy for neural stem cells. In addition, we will discuss ways in which the neurosphere assay has been used to evaluate in vivo treatment/manipulation, and will suggest appropriate ways in which neurosphere data should be interpreted, vis-A-vis the neural stem cell. Finally, we will discuss it relatively new in vitro approach, the Neural-Colony Forming Cell Assay, which provides a more meaningful method of quantifying bona fide neural stem cells without conflating them with more growth-restricted progenitor cells.
Keyword Biochemistry & Molecular Biology
Pharmacology & Pharmacy
neural stem cell
stem cell assay
Subventricular Zone
Neurogenic Regions
Human Brain
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Excellence in Research Australia (ERA) - Collection
Queensland Brain Institute Publications
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Citation counts: TR Web of Science Citation Count  Cited 41 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 47 times in Scopus Article | Citations
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Created: Mon, 18 Feb 2008, 14:20:36 EST