Effect of cytokine and antigen stimulation on peripheral blood lymphocyte syndecan-1 expression

Manakil, J. F., Seymour, G. J. and Bartold, P. M. (2007) Effect of cytokine and antigen stimulation on peripheral blood lymphocyte syndecan-1 expression. Oral Microbiology and Immunology, 22 4: 272-276. doi:10.1111/j.1399-302X.2007.00356.x


Author Manakil, J. F.
Seymour, G. J.
Bartold, P. M.
Title Effect of cytokine and antigen stimulation on peripheral blood lymphocyte syndecan-1 expression
Journal name Oral Microbiology and Immunology   Check publisher's open access policy
ISSN 0902-0055
Publication date 2007
Sub-type Article (original research)
DOI 10.1111/j.1399-302X.2007.00356.x
Volume 22
Issue 4
Start page 272
End page 276
Total pages 5
Editor J. Slots
Place of publication Oxford
Publisher Blackwell Publishing
Collection year 2008
Language eng
Subject C1
730112 Oro-dental and disorders
320899 Dentistry not elsewhere classified
Formatted abstract
Introduction: Cytokines are not only produced by activated lymphocytes but also interact with a number of cell-surface molecules on the same cells. Syndecan-1 is one such cell-surface molecule, which has the capacity to bind a variety of growth factors as well as cytokines. The aim of this study was to examine the effects of transforming growth factor β (TGF-β), interleukin-1 (IL-1), IL-2, IL-4, lipopolysaccharide (LPS) from Porphyromonas gingivalis and tetanus toxoid on syndecan-1 expression by B and T lymphocytes.

Methods: B and T lymphocytes were obtained from the peripheral blood of healthy donors. Following exposure to the above growth factors, cytokines and antigens, syndecan-1 expression was determined by flow cytometry.

Results: Subjects could be categorized as high or low expressors of syndecan-1. In the high-responder group TGF-β1 alone resulted in a significant increase in syndecan-1 expression by both B and T cells. None of the other cytokines and antigens produced a significant response. When analysed in combination, TGF-β1 in combination with IL-2, IL-4, P. gingivalis LPS and tetanus toxoid all produced significant increases in syndecan-1 expression by B cells. For T cells, combinations of TGF-β1 with IL-2 and tetanus toxoid resulted in increased syndecan-1 expression.

Conclusions: Both B and T lymphocytes synthesize the cell-surface proteoglycan syndecan-1 and its expression can be modulated by TGF-β1, either alone or in combination with IL-2, IL-4 and LPS from P. gingivalis and tetanus toxoid. While these may reflect general responses under inflammatory conditions their biological significance requires further investigation.
Keyword Dentistry, Oral Surgery & Medicine
Immunology
Microbiology
lymphocytes
periodontal disease
proteoglycans
syndecan-1
Necrosis-factor-alpha
B-lymphocytes
Tgf-beta
T-cells
Growth-factor
Periodontal-disease
Proteoglycan Synthesis
Signal-transduction
Endothelial-cell
Scid Mice
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2008 Higher Education Research Data Collection
School of Dentistry Publications
 
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Created: Mon, 18 Feb 2008, 17:36:30 EST