A rapid single-step multiplex method for discriminating between Trichogramma (Hymenoptera: Trichogrammatidae) species in Australia

Davies, A. P., Lange, C. L. and O'Neill, S. L. (2006) A rapid single-step multiplex method for discriminating between Trichogramma (Hymenoptera: Trichogrammatidae) species in Australia. Journal of Economic Entomology, 99 6: 2142-2145.


Author Davies, A. P.
Lange, C. L.
O'Neill, S. L.
Title A rapid single-step multiplex method for discriminating between Trichogramma (Hymenoptera: Trichogrammatidae) species in Australia
Journal name Journal of Economic Entomology  (ERA 2012 Listed)    (ERA 2010 Rank B)   Check publisher's open access policy
Publication date 2006-12
Sub-type Article
DOI 10.1603/0022-0493-99.6.2142
Volume number 99
Issue number 6
ISSN 0022-0493
Start page 2142
End page 2145
Total pages 4
Editor John T. Trumble
Alan Kahan
Place of publication Lanham
Publisher Entomological Society America
Language eng
Subject 270307 Microbial Ecology
0703 Crop and Pasture Production
Abstract Inaccurate species identification confounds insect ecological studies. Examining aspects of Trichogramma ecology pertinent to the novel insect resistance management strategy for future transgenic cotton, Gossypium hirsutum L., production in the Ord River Irrigation Area (ORIA) of Western Australia required accurate differentiation between morphologically similar Trichogramma species. Established molecular diagnostic methods for Trichogramma identification use species-specific sequence difference in the internal transcribed spacer (ITS)-2 chromosomal region; yet, difficulties arise discerning polymerase chain reaction (PCR) fragments of similar base pair length by gel electrophoresis. This necessitates the restriction enzyme digestion of PCR-amplified ITS-2 fragments to readily differentiate Trichogramma australicum Girault and Trichogramma pretiosum Riley. To overcome the time and expense associated with a two-step diagnostic procedure, we developed a “one-step” multiplex PCR technique using species-specific primers designed to the ITS-2 region. This approach allowed for a high-throughput analysis of samples as part of ongoing ecological studies examining Trichogramma biological control potential in the ORIA where these two species occur in sympatry.
Keyword Trichogramma australicum
Trichogramma pretiosum
internal transcribed spacer-2
identification
Q-Index Code C1
Additional Notes An author version of the full text will be made available when the Entomological Society of America's 2-year embargo on full text availability ends.

 
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