Nitrifying bacterial communities in an aquaculture wastewater treatment system using fluorescence in situ hybridization (FISH), 16S rRNA gene cloning, and phylogenetic analysis

Paungfoo, Chanyarat, Prasertsan, Poonsuk, Burrell, Paul C., Intrasungkha, Nugul and Blackall, Linda L. (2007) Nitrifying bacterial communities in an aquaculture wastewater treatment system using fluorescence in situ hybridization (FISH), 16S rRNA gene cloning, and phylogenetic analysis. Biotechnology and Bioengineering, 97 4: 985-990. doi:10.1002/bit.21270


Author Paungfoo, Chanyarat
Prasertsan, Poonsuk
Burrell, Paul C.
Intrasungkha, Nugul
Blackall, Linda L.
Title Nitrifying bacterial communities in an aquaculture wastewater treatment system using fluorescence in situ hybridization (FISH), 16S rRNA gene cloning, and phylogenetic analysis
Journal name Biotechnology and Bioengineering   Check publisher's open access policy
ISSN 0006-3592
Publication date 2007-07-01
Sub-type Article (original research)
DOI 10.1002/bit.21270
Volume 97
Issue 4
Start page 985
End page 990
Total pages 6
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Collection year 2008
Language eng
Subject 270301 Bacteriology
300703 Aquaculture
C1
630303 Aquaculture
780105 Biological sciences
Abstract Aquaculture, especially shrimp farming, has played a major role in the growth of Thailand's economy in recent years, as well as in many South East Asian countries. However, the nutrient discharges from these activities have caused adverse impacts on the quality of the receiving waterways. In particular nitrogenous compounds, which may accumulate in aquaculture ponds, can be toxic to aquatic animals and cause environmental problems such as eutrophication. The mineralization process is well known, but certain aspects of the microbial ecology of nitrifiers, the microorganisms that convert ammonia to nitrate; are poorly understood. A previously reported enrichment of nitrifying bacteria (ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB)) from a shrimp farm inoculated in a sequencing batch reactor (SBR) was studied by molecular methods: The initial identification and partial quantification of the nitrifying bacteria (AOB and NOB) were carried out by fluorescence in situ hybridization (FISH) using previously published 16S rRNA-targeting oligonucleotide probes: The two dominant bacterial groups detected by FISH were from the Cytophaga-Flavobacterium-Bacteroides and Proteobacteria (beta subdivision) phyla. Published FISH probes for Nitrobacter and Nitrospira did not hybridize to any of the bacterial cells. Therefore it is likely that new communities of NOBs, differing from previously reported ones, exist in the enrichments. Molecular genetic techniques (cloning, sequencing, and phylogenetic analysis) targeting the 16S rRNA genes from the nitrifying enrichments were performed to identify putative AOBs and NOBs. (C) 2006 Wiley Periodicals, Inc.
Keyword Biotechnology & Applied Microbiology
Fluorescence in situ hybridization (FISH)
Nitrifying bacteria
Phylogenetic analysis
Sequencing batch reactor (SBR)
Shrimp farm
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2008 Higher Education Research Data Collection
School of Chemistry and Molecular Biosciences
 
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Created: Mon, 18 Feb 2008, 17:27:33 EST