Inhibition of airway Na+ transport by respiratory syncytial virus

Kunzelmann, Karl, Sun, Jane, Meanger, Jayesh, King, Nicholas J. and Cook, David I. (2007) Inhibition of airway Na+ transport by respiratory syncytial virus. Journal of Virology, 81 8: 3714-3720. doi:10.1128/JVI.02621-06

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Author Kunzelmann, Karl
Sun, Jane
Meanger, Jayesh
King, Nicholas J.
Cook, David I.
Title Inhibition of airway Na+ transport by respiratory syncytial virus
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
Publication date 2007-04-01
Year available 2007
Sub-type Article (original research)
DOI 10.1128/JVI.02621-06
Open Access Status File (Publisher version)
Volume 81
Issue 8
Start page 3714
End page 3720
Total pages 7
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2008
Language eng
Subject C1
329999 Medical and Health Sciences not elsewhere classified
780105 Biological sciences
Abstract In previous studies, we have shown that two major respiratory pathogens, influenza virus and parainfluenza virus, produce acute alterations in ion transport upon contacting the apical membrane of the respiratory epithelium. In the present study, we examine the effects on ion transport by the mouse tracheal epithelium of a third major respiratory pathogen, respiratory syncytial virus (RSV). RSV infections are associated with fluid accumulation in the respiratory tract and cause illnesses that range in severity from rhinitis, sinusitis, otitis media, and bronchitis to bronchiolitis and pneumonia. We find that within minutes of RSV contacting the apical membrane; it inhibits amiloride-sensitive Na+ transport by the epithelium. This effect is mediated by protein kinase C and is reproduced by recombinant viral F (fusion) protein. Since this inhibition is not accompanied by any alteration in the epithelial responses to carbachol or to forskolin plus 3-isobutyl-1-methylxanthine (IBMX), it is not due to a nonspecific toxic action of the virus. The inhibition also appears to require Toll-like receptor 4 and the presence of asialogangliosides in the apical membrane. Since the concentration range over which this inhibition is observed (10(2) to 10(5) PFU/ml) is comparable to the viral concentrations observed in clinical and experimental RSV infections, it seems likely that direct inhibition by the virus of epithelial Na+ transport may contribute to the fluid accumulation that is observed in RSV infections.
Keyword Virology
Bioelectric Properties
Tracheal Epithelium
Host Response
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2008 Higher Education Research Data Collection
School of Biomedical Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 17 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 18 times in Scopus Article | Citations
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Created: Mon, 18 Feb 2008, 16:51:17 EST