NMR as a tool for elucidating the structures of circular and knotted proteins

Craik, D. J and Daly, N. L (2007) NMR as a tool for elucidating the structures of circular and knotted proteins. Molecular Biosystems, 3 4: 257-265. doi:10.1039/b616856f


Author Craik, D. J
Daly, N. L
Title NMR as a tool for elucidating the structures of circular and knotted proteins
Journal name Molecular Biosystems   Check publisher's open access policy
ISSN 1742-206X
Publication date 2007
Year available 2007
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1039/b616856f
Open Access Status Not Open Access
Volume 3
Issue 4
Start page 257
End page 265
Total pages 9
Place of publication Cambridge, United Kingdom
Publisher Royal Society of Chemistry
Collection year 2008
Language eng
Abstract Cyclotides are a recently discovered family of mini-proteins that have a head-to-tail cyclised backbone stabilized by a knotted arrangement of three disulfide bonds. They have a wide range of biological activities, including uterotonic, anti-bacterial, anti-HIV, and anti-tumour activity but their insecticidal activities suggest that their natural function is in plant defense. They are exceptionally resistant to chemical, enzymatic and thermal treatments because of their unique structural scaffold. This stability and resistance to proteolysis makes them a potentially valuable protein engineering tool at the interface of chemistry and biology: they have the structure of proteins but the stability and biophysical properties of organic molecules. In this review the role of NMR in defining the structures of cyclotides is described.
Keyword Biochemistry & Molecular Biology
Cyclic-cystine-knot
Inhibitory Macrocyclic Peptides
Cyclotide Cycloviolacin O2
Disulfide Folding Pathways
Polypeptide Kalata B1
Anti-hiv Activity
Trypsin-inhibitor
Drug Design
Momordica-cochinchinensis
Backbone Cyclization
Q-Index Code C1
Q-Index Status Confirmed Code
Additional Notes This document is a journal review.

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Excellence in Research Australia (ERA) - Collection
2008 Higher Education Research Data Collection
Institute for Molecular Bioscience - Publications
 
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Created: Mon, 18 Feb 2008, 16:49:05 EST