Microbial diversity within early-stage cultured Panulirus ornatus phyllosomas

Payne, MS, Hall, MR, Sly, L and Bourne, DG (2007) Microbial diversity within early-stage cultured Panulirus ornatus phyllosomas. Applied And Environmental Microbiology, 73 6: 1940-1951. doi:10.1128/AEM.02520-06

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
UQ128334_OA.pdf Full text (open access) application/pdf 2.65MB 0

Author Payne, MS
Hall, MR
Sly, L
Bourne, DG
Title Microbial diversity within early-stage cultured Panulirus ornatus phyllosomas
Formatted title
Microbial diversity within early-stage cultured Panulirus ornatus phyllosomas
Journal name Applied And Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
Publication date 2007-01-12
Sub-type Article (original research)
DOI 10.1128/AEM.02520-06
Open Access Status File (Publisher version)
Volume 73
Issue 6
Start page 1940
End page 1951
Total pages 12
Place of publication Washington
Publisher Amer Soc Microbiology
Collection year 2008
Language eng
Subject 270307 Microbial Ecology
270504 Invertebrate Biology
270702 Marine and Estuarine Ecology (incl. Marine Ichthyology)
630303 Aquaculture
Abstract A thorough understanding of the microorganisms and pathogens associated with the larval stage of the tropical ornate rock lobster, Panulirus ornatus, is required to overcome disease outbreaks that currently block aquaculture attempts. This study used microscopy in addition to culture and molecularly based microbiological techniques to characterize the bacterial community associated with cultured, developmental stage PI to II P. ornatus phyllosomas. Scanning electron microscopy demonstrated colonization of phyllosomas by filamentous, rod-shaped, and coccus-shaped bacteria. A clone library constructed from dead phyllosomas sampled from the larval rearing tank on day 10 was dominated by Thiothrix-affiliated sequences (56% of clones). A comparable library from live phyllosomas also contained Thiothrix-affiliated sequences, though these only represented 19% of clones within the library. Fluorescent in situ hybridization (FISH) confirmed identification of the filamentous bacteria as Thiothrix sp., being present on dead phyllosomas. FISH also identified Leucothrix sp. and ribrio sp., as well as a range of other rod- and coccus-shaped bacteria, colonizing both live and dead phyllosomas. The development of the microbial community associated with phyllosomas was monitored through-a standard larval rearing run using denaturing gradient gel electrophoresis (DGGE). Vibrio sp.-affiliated bands dominated the profiles of live animals through the rearing period and dead phyllosomas sampled on selected days. The population of Vibrio sp. associated with phyllosomas was monitored with culture-based analysis on selective media and demonstrated to increase significantly on day 7, coinciding with the beginning of the larval molt. An isolated Vibrio harveyi strain demonstrated an identical 16S rRNA sequence with retrieved DGGE and clone library sequences. Colonization of phyllosomas with filamentous bacterial species potentially hinders the ability of the animals to molt and, combined with the added stress of the molt process, likely results in reduced immune function, allowing opportunistic pathogenic Vibrio sp. to cause larval mortalities.
Keyword Biotechnology & Applied Microbiology
16s Ribosomal-rna
Gradient Gel-electrophoresis
Luminous Vibrio-harveyi
Tropical Rock Lobster
Phylogenetic Analysis
Hypercapnic Hypoxia
Q-Index Code C1
Q-Index Status Confirmed Code

Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 24 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 24 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 18 Feb 2008, 16:42:44 EST