A polymerase chain reaction-based diagnostic to identify larvae and eggs of container mosquito species from the Australian region

Beebe, Nigel W., Whelan, Peter I., van den Hurk, Andrew F., Ritchie, Scott A., Corcoran, Sarah and Cooper, Robert D. (2007) A polymerase chain reaction-based diagnostic to identify larvae and eggs of container mosquito species from the Australian region. Journal of Medical Entomology, 44 2: 376-380. doi:10.1603/0022-2585(2007)44[376:APCRDT]2.0.CO;2


Author Beebe, Nigel W.
Whelan, Peter I.
van den Hurk, Andrew F.
Ritchie, Scott A.
Corcoran, Sarah
Cooper, Robert D.
Title A polymerase chain reaction-based diagnostic to identify larvae and eggs of container mosquito species from the Australian region
Journal name Journal of Medical Entomology   Check publisher's open access policy
ISSN 0022-2585
Publication date 2007-01-01
Sub-type Article (original research)
DOI 10.1603/0022-2585(2007)44[376:APCRDT]2.0.CO;2
Volume 44
Issue 2
Start page 376
End page 380
Total pages 5
Place of publication Lanham
Publisher Entomological Society America
Collection year 2008
Language eng
Subject 270504 Invertebrate Biology
270203 Population and Ecological Genetics
780105 Biological sciences
C1
730101 Infectious diseases
Abstract Dengue outbreaks occur regularly in parts of northern Queensland, Australia, and there is Concern that these outbreaks may spread with the introduction and range expansion of the two main vectors Aedcs aegypti (L.) and Aedes albopictus (Skuse). Problems encountered in separating larvae of endemic and exotic container mosquito species resulted in the development of a polymerase chain reaction diagnostic procedure that uses a restriction enzyme to cut the internal transcribed spacer region 1 of the ribosomal DNA to separate Ae. aegypti and Ae. albopictus from a number of common local container mosquito species which can be used at any stage of the life cycle, including eggs up to 8 wk of age. Identification was possible using desiccated or alcohol-preserved specimens with a response time of < 24 h after receipt of the specimens.
Keyword Entomology
Veterinary Sciences
polymerase chain reaction diagnostic
container mosquito species
internal transcribed spacer region 1
Aedes mosquitoes
Aedes-albopictus
Q-Index Code C1
Q-Index Status Confirmed Code

 
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