A method for estimating the nature and relative proportions of amorphous, single, and double-helical components in starch granules by 13C CP/MAS NMR

Tan, Ihwa, Flanagan, Bernadine M., Halley, Peter J., Whittaker, Andrew K. and Gidley, Michael J. (2007) A method for estimating the nature and relative proportions of amorphous, single, and double-helical components in starch granules by 13C CP/MAS NMR. Biomacromolecules, 8 3: 885-891.


Author Tan, Ihwa
Flanagan, Bernadine M.
Halley, Peter J.
Whittaker, Andrew K.
Gidley, Michael J.
Title A method for estimating the nature and relative proportions of amorphous, single, and double-helical components in starch granules by 13C CP/MAS NMR
Formatted title A method for estimating the nature and relative proportions of amorphous, single, and double-helical components in starch granules by 13C CP/MAS NMR
Journal name Biomacromolecules  (ERA 2012 Listed)    (ERA 2010 Rank A)   Check publisher's open access policy
Publication date 2007-03
Sub-type Article
DOI 10.1021/bm060988a
Volume number 8
Issue number 3
ISSN 1525-7797
Start page 885
End page 891
Total pages 7
Editor A. C. Albertsson
Place of publication Washington, D. C., U.S.A.
Publisher American Chemical Society
Collection year 2008
Language eng
Subject 290100 Industrial Biotechnology and Food Sciences
620400 Primary Products From Plants
C1
Abstract An improved method to analyze the C-13 NMR spectra of native starches, which considers the contribution of the V-type conformation and the nature of the amorphous component, has been developed. Starch spectra are separated into amorphous and ordered subspectra, using intensity at 84 ppm as a reference point. The ordered subspectra of high amylose starches show the presence of both V-type single helices and B-type double helices. Relative proportions of amorphous, single, and double-helical conformations are estimated by apportioning intensity of C1 peak areas between conformational types on the basis of ordered and amorphous subspectra of the native starch. Quantitative analysis shows that the V-type single-helical component increases with amylose content of starches. Different amorphous subspectra are needed to provide a consistent analysis of granular starches from diverse sources. The method of preparation was found to be more important than the starch botanical origin in determining C-13 NMR spectral features of amorphous samples.
Keyword Biochemistry & Molecular Biology
Chemistry, Organic
Polymer Science
Amylose Inclusion Complexes
V-amylose
Molecular-structure
Packing Analysis
Crystalline-a
Spectroscopy
Polysaccharides
Polymorphs
Scattering
Maize
Q-Index Code C1
Q-Index Status Confirmed Code

 
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Created: Mon, 18 Feb 2008, 16:33:46 EST