A multicopper oxidase is essential for manganese oxidation and laccase-like activity in Pedomicrobium sp ACM 3067

Ridge, Justin P., Lin, Marianne, Larsen, Eloise I., Fegan, Mark, McEwan, Alastair G. and Sly, Lindsay I. (2007) A multicopper oxidase is essential for manganese oxidation and laccase-like activity in Pedomicrobium sp ACM 3067. Environmental Microbiology, 9 4: 944-953.


Author Ridge, Justin P.
Lin, Marianne
Larsen, Eloise I.
Fegan, Mark
McEwan, Alastair G.
Sly, Lindsay I.
Title A multicopper oxidase is essential for manganese oxidation and laccase-like activity in Pedomicrobium sp ACM 3067
Formatted title A multicopper oxidase is essential for manganese oxidation and laccase-like activity in Pedomicrobium sp ACM 3067
Journal name Environmental Microbiology  (ERA 2012 Listed)    (ERA 2010 Rank A*)   Check publisher's open access policy
Publication date 2007-04
Sub-type Article
Year available 2006
DOI 10.1111/j.1462-2920.2006.01216.x
Volume number 9
Issue number 4
ISSN 1462-2912
Start page 944
End page 953
Total pages 10
Place of publication Oxford, England
Publisher Blackwell Publishing
Collection year 2008
Language eng
Subject C1
270306 Microbial Genetics
270301 Bacteriology
780105 Biological sciences
Abstract Pedomicrobium sp. ACM 3067 is a budding-hyphal bacterium belonging to the alpha-Proteobacteria which is able to oxidize soluble Mn2+ to insoluble manganese oxide. A cosmid, from a whole-genome library, containing the putative genes responsible for manganese oxidation was identified and a primer-walking approach yielded 4350 bp of novel sequence. Analysis of this sequence showed the presence of a predicted three-gene operon, moxCBA. The moxA gene product showed homology to multicopper oxidases (MCOs) and contained the characteristic four copper-binding motifs (A, B, C and D) common to MCOs. An insertion mutation of moxA showed that this gene was essential for both manganese oxidation and laccase-like activity. The moxB gene product showed homology to a family of outer membrane proteins which are essential for Type I secretion in Gram-negative bacteria. moxBA has not been observed in other manganese-oxidizing bacteria but homologues were identified in the genomes of several bacteria including Sinorhizobium meliloti 1021 and Agrobacterium tumefaciens C58. These results suggest that moxBA and its homologues constitute a family of genes encoding an MCO and a predicted component of the Type I secretion system.
Keyword Microbiology
Leptothrix-discophora Ss-1
Multi-copper Oxidase
Microbial Oxidation
Oxidizing Factor
Outer-membrane
Mn2+ Oxidation
Gene
Iron
Identification
Manganicum
Q-Index Code C1
Q-Index Status Confirmed Code

 
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Created: Mon, 18 Feb 2008, 16:32:36 EST