Differential changes in Plasmodium falciparum var transcription during adaptation to culture

Peters, Jennifer M., Fowler, Elizabeth V., Krause, Darren R., Cheng, Qin and Gatton, Michelle L. (2007) Differential changes in Plasmodium falciparum var transcription during adaptation to culture. Journal of Infectious Diseases, 195 5: 748-755. doi:10.1086/511436


Author Peters, Jennifer M.
Fowler, Elizabeth V.
Krause, Darren R.
Cheng, Qin
Gatton, Michelle L.
Title Differential changes in Plasmodium falciparum var transcription during adaptation to culture
Journal name Journal of Infectious Diseases   Check publisher's open access policy
ISSN 0022-1899
1537-6613
Publication date 2007
Sub-type Article (original research)
DOI 10.1086/511436
Volume 195
Issue 5
Start page 748
End page 755
Total pages 8
Place of publication Cary, NC, United States
Publisher Oxford University Press
Collection year 2008
Language eng
Abstract Plasmodium falciparum erythrocyte membrane protein 1, which is encoded by the var multigene family, is expressed on the surface of P. falciparum-infected erythrocytes and has been implicated in many of the complications associated with falciparum malaria. Transcriptional switching of var is commonly investigated using in vitro cultured parasites, because parasite material from patients is limited. We investigated the affect of short-term in vitro cultivation on var gene transcription in patient samples. A significant reduction in the overall abundance of var transcripts was observed during the first similar to 10 days of culture. The rate of down-regulation was not constant among all var genes; genes with an upsA, -D, and -E 5' flanking region had a significantly faster rate than genes with an upsB or -C flanking region. These results have significant implications for the investigation of associations between var transcription and clinical manifestations using parasites that have been enriched by in vitro culture.
Keyword Infectious Diseases
Chondroitin Sulfate-a
Structured Var Gene
Antigenic Variation
Infected Erythrocytes
Variant Antigen
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2008 Higher Education Research Data Collection
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 28 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 28 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 18 Feb 2008, 16:19:41 EST