From sequence to antibody: Genetic immunisation is suitable to generate antibodies against a rare plant membrane protein, the KAT 1 channel

Gehwolf, Renate, Weiss, Richard, Gabler, Maximilian, Hurst, Annette C., Bertl, Adam, Thalhamer, Josef and Obermeyer, Gerhard (2007) From sequence to antibody: Genetic immunisation is suitable to generate antibodies against a rare plant membrane protein, the KAT 1 channel. Febs Letters, 581 3: 448-452. doi:10.1016/j.febslet.2007.01.004


Author Gehwolf, Renate
Weiss, Richard
Gabler, Maximilian
Hurst, Annette C.
Bertl, Adam
Thalhamer, Josef
Obermeyer, Gerhard
Title From sequence to antibody: Genetic immunisation is suitable to generate antibodies against a rare plant membrane protein, the KAT 1 channel
Journal name Febs Letters   Check publisher's open access policy
ISSN 0014-5793
Publication date 2007-02-06
Sub-type Article (original research)
DOI 10.1016/j.febslet.2007.01.004
Volume 581
Issue 3
Start page 448
End page 452
Total pages 5
Editor Ulf-Ingo Flügge
Place of publication Amsterdam, The Netherlands
Publisher Elsevier Science Bv
Collection year 2008
Language eng
Subject C1
320202 Cellular Immunology
780105 Biological sciences
Abstract Monoclonal antibodies against the K+ channel KAT1 of Arabidopsis thaliana, a low abundance, plant plasma membrane protein, were generated by genetic immunisation to avoid the time and labour consuming purification of native or recombinant proteins and peptides usually necessary for conventional immunisation techniques. The resulting polyclonal and monoclonal antibody sera recognised a single protein band in a microsomal fraction of wild-type A. thaliana leaves and in membrane fractions of transgenic yeast cells and tobacco plants expressing the KAT1 protein. Therefore, genetic immunisation is suitable for generating monoclonal antibodies against plant proteins and particularly, against plant membrane proteins of low abundance. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Keyword Biochemistry & Molecular Biology
Biophysics
Cell Biology
genetic immunisation
K+ channel
monoclonal antibody
plasma membrane protein
Potassium Channel
Plasma-membrane
Dna Vaccines
K+ Channel
Circumsporozoite Protein
Arabidopsis-thaliana
Malaria Infection
Immune-responses
Expression
Cells
Q-Index Code C1
Q-Index Status Confirmed Code

 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in Thomson Reuters Web of Science Article
Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Mon, 18 Feb 2008, 16:19:36 EST