Lamellar pro-inflammatory cytokine expression patterns in laminitis at the developmental stage and at the onset of lameness: Innate vs. adaptive immune response

Belknap, J. K., Giguere, S., Pettigrew, A., Cochran, A. M., Van Eps, A. W. and Pollitt, C. C. (2007) Lamellar pro-inflammatory cytokine expression patterns in laminitis at the developmental stage and at the onset of lameness: Innate vs. adaptive immune response. Equine Veterinary Journal, 39 1: 42-47. doi:10.2746/042516407X155406


Author Belknap, J. K.
Giguere, S.
Pettigrew, A.
Cochran, A. M.
Van Eps, A. W.
Pollitt, C. C.
Title Lamellar pro-inflammatory cytokine expression patterns in laminitis at the developmental stage and at the onset of lameness: Innate vs. adaptive immune response
Journal name Equine Veterinary Journal   Check publisher's open access policy
ISSN 0425-1644
2042-3306
Publication date 2007-01-01
Sub-type Article (original research)
DOI 10.2746/042516407X155406
Volume 39
Issue 1
Start page 42
End page 47
Total pages 6
Place of publication Newmarket, U.K.
Publisher Equine Veterinary Journal Ltd
Language eng
Formatted abstract
Reasons for performing study: Recent research has indicated that inflammation plays a role in the early stages of laminitis and that, similar to organ failure in human sepsis, early inflammatory mechanisms may lead to downstream events resulting in lamellar failure. Characterisation of the type of immune response (i.e. innate vs. adaptive) is essential in order to develop therapeutic strategies to counteract these deleterious events.
Objectives: To quantitate gene expression of pro-inflammatory cytokines known to be important in the innate and adaptive immune response during the early stages of laminitis, using both the black walnut extract (BWE) and oligofructose (OF) models of laminitis.
Methods: Real-time qPCR was used to assess lamellar mRNA expression of interleukins-1 beta, 2, 4, 6, 8, 10, 12 and 18, and tumour necrosis factor alpha and interferon gamma at the developmental stage and at the onset of lameness.
Results: Significantly increased lamellar mRNA expression of cytokines important in the innate immune response were present at the developmental stage of the BWE model, and at the onset of acute lameness in both the BWE model and OF model. Of the cytokines characteristic of the Th1 and Th2 arms of the adaptive immune response, a mixed response was noted at the onset of acute lameness in the BWE model, whereas the response was skewed towards a Th1 response at the onset of lameness in the OF model.
Conclusions: Lamellar inflammation is characterised by strong innate immune response in the developmental stages of laminitis; and a mixture of innate and adaptive immune responses at the onset of lameness.
Potential relevance: These results indicate that antiinflammatory treatment of early stage laminitis (and the horse at risk of laminitis) should include not only therapeutic drugs that address prostanoid activity, but should also address the marked increases in lamellar cytokine expression.
Keyword Veterinary Sciences
Horse
Laminitis
Cytokine
Real time-quantitative PCR (RT-qPCR)
Immune response
Respiratory-distress-syndrome
Inducible Nuclear-protein
Walnut-induced Laminitis
Equine Laminitis
Prodromal Stage
Gene-expression
Sepsis
Horses
Pathophysiology
Interleukin-10
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
 
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Created: Tue, 19 Feb 2008, 02:06:02 EST