Abundance and ecophysiology of Defluviicoccus spp., glycogen-accumulating organisms in full-scale wastewater treatment processes

Burow, Luke C., Kong, Yunhong, Nielsen, Jeppe L., Blackall, Linda L. and Nielsen, Peter H. (2007) Abundance and ecophysiology of Defluviicoccus spp., glycogen-accumulating organisms in full-scale wastewater treatment processes. Microbiology, 153 1: 178-185.


Author Burow, Luke C.
Kong, Yunhong
Nielsen, Jeppe L.
Blackall, Linda L.
Nielsen, Peter H.
Title Abundance and ecophysiology of Defluviicoccus spp., glycogen-accumulating organisms in full-scale wastewater treatment processes
Formatted title Abundance and ecophysiology of Defluviicoccus spp., glycogen-accumulating organisms in full-scale wastewater treatment processes
Journal name Microbiology  (ERA 2012 Listed)    (ERA 2010 Rank A)   Check publisher's open access policy
Publication date 2007
Sub-type Article
DOI 10.1099/mic.0.2006/001032-0
Volume number 153
Issue number 1
ISSN 1350-0872
Start page 178
End page 185
Total pages 8
Place of publication Reading, England
Publisher Society of General Microbiology
Language eng
Subject C1
291100 Environmental Engineering
779999 Other
Formatted abstract The activity of glycogen-accumulating organisms (GAOs) in enhanced biological phosphorus removal (EBPR) wastewater treatment plants has been proposed as one cause of deterioration of EBPR. Putative GAOs from the Alphaproteobacteria, Defluviicoccus spp. (including D. vanus), were studied in full-scale EBPR plants to determine their distribution, abundance and ecophysiology. Fluorescence in situ hybridization (FISH) demonstrated that Defluviicoccus spp. were generally low in abundance; however, in one plant surveyed, Cluster 2 Defluviicoccus constituted 9 % of all Bacteria. FISH combined with microautoradiography revealed that both Cluster 1 and Cluster 2 Defluviicoccus were capable of taking up a narrow range of substrates including acetate, propionate, pyruvate and glucose under anaerobic and aerobic conditions. Formate, butyrate, ethanol and several other substrates were not taken up. Cluster 2 Defluviicoccus demonstrated a phenotype consistent with the current metabolic model for GAOs - anaerobic assimilation of acetate and reduction to polyhydroxyalkanoates (PHA) using the glycolytic pathway, and aerobic consumption of PHA. Polyphosphate-accumulating organisms (PAOs, 'Candidatus Accumulibacter phosphatis') and other putative GAOs ('Candidatus Competibacter phosphatis') co-existed in two plants with Cluster 2 Defluviicoccus, but in both plants, the latter organisms were more abundant. Thus Ciuster.2 Defluviicoccus can be relatively abundant and could be carbon competitors of PAOs and other GAOs in EBPIR plants.
Keyword Microbiology
Biological Phosphorus Removal
Aerobic Activated-sludge
Targeted Oligonucleotide Probes
In-situ Hybridization
Anaerobic Conditions
Treatment Plants
Substrate Uptake
Metabolic Model
Identification
Bacteria
Q-Index Code C1

 
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