Development and evaluation of real-time polymerase chain reaction assays to identify mosquito (Diptera : Culicidae) bloodmeals originating from native Australian mammals

Van den Hurk, Andrew F., Smith, Ina L. and Smith, Greg A. (2007) Development and evaluation of real-time polymerase chain reaction assays to identify mosquito (Diptera : Culicidae) bloodmeals originating from native Australian mammals. Journal of Medical Entomology, 44 1: 85-92. doi:10.1603/0022-2585(2007)44[85:DAEORP]2.0.CO;2


Author Van den Hurk, Andrew F.
Smith, Ina L.
Smith, Greg A.
Title Development and evaluation of real-time polymerase chain reaction assays to identify mosquito (Diptera : Culicidae) bloodmeals originating from native Australian mammals
Journal name Journal of Medical Entomology   Check publisher's open access policy
ISSN 0022-2585
Publication date 2007-01-01
Sub-type Article (original research)
DOI 10.1603/0022-2585(2007)44[85:DAEORP]2.0.CO;2
Volume 44
Issue 1
Start page 85
End page 92
Total pages 8
Place of publication Lanham, MD, USA
Publisher Entomological Society America
Collection year 2008
Language eng
Subject 270504 Invertebrate Biology
300508 Parasitology
C1
780105 Biological sciences
Abstract Real-time TaqMan polymerase chain reaction (PCR) assays were developed for the identification of mosquito (Diptera: Culicidae) bloodmeals originating from three groups of native Australian mammals. Primers and probes were designed to amplify a partial fragment of the cytochrome b gene of the agile wallaby, Macropus agilis (Gould); brushtail possum, Trichosurus vulpecula (Kerr); and the consensus sequence of the four species of Australian flying fox: Pteropus alecto Temminck, Pteropus conspicillatus Gould, Pteropus poliocephalus Temminck, and Pteropus scapulatus Peters. When tested on DNA extracted from whole tissue, each assay was shown to be specific for the vertebrate host that it was designed to identify. To evaluate the TaqMan assays, 137 field-collected blood-fed mosquitoes were analyzed, from which 128 (93.4%) were identified using one of the assays. Compared with other PCR-based techniques for bloodmeal identification, the TaqMan assays are sensitive, specific, and provide a rapid result without the need for post-PCR manipulation and visualization of products.
Keyword Entomology
Veterinary Sciences
mosquito
bloodmeal
real-time
PCR
identification
Japanese Encephalitis-virus
Linked-immunosorbent-assay
Host-feeding Patterns
Ross River Virus
Cytochrome-b Gene
West-nile-virus
Heteroduplex Analysis
Human-disease
Human Dna
Identification
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2008 Higher Education Research Data Collection
School of Chemistry and Molecular Biosciences
 
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Created: Mon, 18 Feb 2008, 16:01:34 EST