Hepatitis C virus p7 protein is localized in the endoplasmic reticulum when it is encoded by a replication-competent genome

Haqshenas, G., Mackenzie, J. M., Dong, X. and Gowans, E. J. (2007) Hepatitis C virus p7 protein is localized in the endoplasmic reticulum when it is encoded by a replication-competent genome. Journal of General Virology, 88 1: 134-142. doi:10.1099/vir.0.82049-0


Author Haqshenas, G.
Mackenzie, J. M.
Dong, X.
Gowans, E. J.
Title Hepatitis C virus p7 protein is localized in the endoplasmic reticulum when it is encoded by a replication-competent genome
Journal name Journal of General Virology   Check publisher's open access policy
ISSN 0022-1317
Publication date 2007-01
Year available 2006
Sub-type Article (original research)
DOI 10.1099/vir.0.82049-0
Volume 88
Issue 1
Start page 134
End page 142
Total pages 9
Place of publication Reading, England
Publisher Soc General Microbiology
Collection year 2008
Language eng
Subject 270303 Virology
320402 Medical Virology
C1
730101 Infectious diseases
Abstract p7 protein is a small protein encoded by Hepatitis C virus (HCV) that functions as an ion channel in planar lipid bilayers. The function of p7 is vital for the virus life cycle. In this study, the p7 protein of genotype 2a (strain JFH1; the only strain that replicates and produces virus progeny in vitro) was tagged with either an enhanced green fluorescent protein (eGFP) or a haemagglutinin (HA) epitope to facilitate tracking of the protein in the intracellular environment. The tagged viral polyprotein was expressed transiently in the cells after transfection with the recombinant RNA transcripts. Confocal microscopy revealed that the tagged p7 protein was localized in the endoplasmic reticulum (ER) but not associated with mitochondria. Immunoelectron microscopy confirmed the p7 localization data and, moreover, showed that intracellular virus-like particles formed in the cells transfected with the wild-type, but not the recombinant, transcripts. Following a few passages of the transfected cells, the recombinant genome with the HA tag reverted to wild-type and the entire tag was deleted. Therefore, in this study, it has been demonstrated that the p7 protein in the context of the full-length polyprotein encoded by a replication competent genome is only localized to the ER and has a possible role in HCV particle formation.
Keyword Biotechnology & Applied Microbiology
Virology
Ion-channel Activity
Cell-culture
Subcellular-localization
Mammalian-cells
Tissue-culture
In-vitro
Identification
Polypeptide
Amantadine
Infection
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2008 Higher Education Research Data Collection
School of Chemistry and Molecular Biosciences
 
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Created: Mon, 18 Feb 2008, 16:01:21 EST