Spatio-temporal characterization of polyhydroxybutyrate accumulation in sugarcane

Purnell, Matthew P., Petrasovits, Lars A., Nielsen, Lars K. and Brumbley, Stevens M. (2007) Spatio-temporal characterization of polyhydroxybutyrate accumulation in sugarcane. Plant Biotechnology Journal, 5 1: 173-184. doi:10.1111/j.1467-7652.2006.00230.x


Author Purnell, Matthew P.
Petrasovits, Lars A.
Nielsen, Lars K.
Brumbley, Stevens M.
Title Spatio-temporal characterization of polyhydroxybutyrate accumulation in sugarcane
Journal name Plant Biotechnology Journal   Check publisher's open access policy
ISSN 1467-7644
Publication date 2007
Sub-type Article (original research)
DOI 10.1111/j.1467-7652.2006.00230.x
Volume 5
Issue 1
Start page 173
End page 184
Total pages 12
Place of publication Oxford
Publisher Blackwell Publishing
Collection year 2008
Language eng
Subject 270899 Biotechnology not elsewhere classified
C1
780105 Biological sciences
Abstract We report here the results from a glasshouse trial of several transgenic sugarcane (Saccharum spp. hybrids) lines accumulating the bacterial polyester polyhydroxybutyrate (PHB) in plastids. The aims of the trial were to characterize the spatio-temporal pattern of PHB accumulation at a whole-plant level, to identify factors limiting PHB production and to determine whether agronomic performance was affected adversely by PHB accumulation. Statistical analysis showed that a vertical PHB concentration gradient existed throughout the plant, the polymer concentration being lowest in the youngest leaves and increasing with leaf age. In addition, there was a horizontal gradient along the length of a leaf, with the PHB concentration increasing from the youngest part of the leaf (the base) to the oldest (the tip). The rank order of the lines did not change over time. Moreover, there was a uniform spatio-temporal pattern of relative PHB accumulation among the lines, despite the fact that they showed marked differences in absolute PHB concentration. Molecular analysis revealed that the expression of the transgenes encoding the PHB biosynthesis enzymes was apparently coordinated, and that there were good correlations between PHB concentration and the abundance of the PHB biosynthesis enzymes. The maximum recorded PHB concentration, 1.77% of leaf dry weight, did not confer an agronomic penalty. The plant height, total aerial biomass and culm-internode sugar content were not affected relative to controls. Although moderate PHB concentrations were achieved in leaves, the maximum total-plant PHB yield was only 0.79% (11.9 g PHB in 1.51 kg dry weight). We combine the insights from our statistical and molecular analyses to discuss possible strategies for increasing the yield of PHB in sugarcane.
Keyword Biotechnology & Applied Microbiology
Plant Sciences
biofactory
polyhydroxybutyrate
real-time polymerase chain reaction
Saccharum
transgenic sugarcane
Poly-beta-hydroxybutyrate
Alcaligenes-eutrophus H16
Transplastomic Tobacco
Transgenic Tobacco
Expression
Plants
Gene
Ketothiolase
Arabidopsis
Biosynthesis
Q-Index Code C1
Q-Index Status Confirmed Code

 
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Created: Mon, 18 Feb 2008, 15:57:20 EST