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A molecular approach to the identification of cytotoxic T-lymphocyte epitopes within equine herpesvirus 1
Kydd, J. H., Davis-Poynter, N. J., Birch, J., Hannant, D., Minke, J., Audonnet, J. C., Antczak, D. F. and Ellis, S. A. (2006-09) A molecular approach to the identification of cytotoxic T-lymphocyte epitopes within equine herpesvirus 1. Journal of General Virology, 87 9: 2507-2515.
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| Author(s) |
Kydd, J. H.
Davis-Poynter, N. J.
Birch, J.
Hannant, D.
Minke, J.
Audonnet, J. C.
Antczak, D. F.
Ellis, S. A.
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| Title |
A molecular approach to the identification of cytotoxic T-lymphocyte epitopes within equine herpesvirus 1
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| Journal name |
Journal of General Virology
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| Publication date |
2006-09
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| Volume number |
87
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| Issue number |
9
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| ISSN |
0022-1317
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| Start page |
2507
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| End page |
2515
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| Total pages |
9
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| Place of publication |
Reading, England
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| Publisher |
Society for General Microbiology
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| Language |
eng
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| Subject |
060506 Virology
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| Abstract |
Equine herpesvinus 1 (EHV-1) causes respiratory and neurological disease and abortion in horses. Animals with high frequencies of cytotoxic T lymphocytes (CTL) show reduced severity of respiratory disease and frequency of abortion, probably by CTL-mediated control of cell-associated viraemia. This study aimed to identify CTL epitopes restricted by selected major histocompatibility complex (MHC) class I alleles expressed in the equine leukocyte antigen (ELA) A3 haplotype. Effector CTL were induced from EHV-1-primed ponies and thoroughbreds with characterized MHC class I haplotypes and screened against P815 target cells transfected with selected EHV-1 genes and MHC class I genes. Targets that expressed EHV-1 gene 64 and the MHC B2 gene were lysed by effector CTL in a genetically restricted manner. There was no T-cell recognition of targets expressing either the MHC B2 gene and EHV-1 genes 2, 12, 14, 16, 35, 63 or 69, or the MHC C1 gene and EHV-1 genes 12, 14, 16 or 64. A vaccinia virus vector encoding gene 64 (NYVAC-64) was also investigated. Using lymphocytes from ELA-A3 horses, the recombinant NYVAC-64 virus induced effector CTL that lysed EHV-1-infected target cells; the recombinant virus also supplied a functional peptide that was expressed by target cells and recognized in an MHC-restricted fashion by CTL induced with EHV-1. This construct may therefore be used to determine the antigenicity of EHV-1 gene 64 for other MHC haplotypes. These techniques are broadly applicable to the identification of additional CTL target proteins and their presenting MHC alleles, not only for EHV-1, but for other equine viruses.
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| Keyword(s) |
Biotechnology & Applied Microbiology
Virology
Major Histocompatibility Complex
Vaccinia Virus
Pregnant Mares
Dna-sequence
Horse
Type-1
Immunosuppression
Alloantigens
Frequencies
Expression
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