Inhibition of the proteolytic activity of indigenous plasmin or exogenous chymosin and pepsin in bovine milk by blood serum

Huppertz, Thom, Uniacke, Therese, Kelly, Alan L. and Fox, Patrick F. (2006) Inhibition of the proteolytic activity of indigenous plasmin or exogenous chymosin and pepsin in bovine milk by blood serum. International Dairy Journal, 16 6: 691-696. doi:10.1016/j.idairyj.2005.10.022


Author Huppertz, Thom
Uniacke, Therese
Kelly, Alan L.
Fox, Patrick F.
Title Inhibition of the proteolytic activity of indigenous plasmin or exogenous chymosin and pepsin in bovine milk by blood serum
Journal name International Dairy Journal   Check publisher's open access policy
ISSN 0958-6946
Publication date 2006-06
Sub-type Article (original research)
DOI 10.1016/j.idairyj.2005.10.022
Volume 16
Issue 6
Start page 691
End page 696
Total pages 6
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract Adding bovine or ovine blood serum (0.5-2.0%, v/v) to bovine milk did not affect the hydrolysis of caseins by plasmin, but equine, and particularly porcine, serum strongly inhibited casein hydrolysis.- in milk containing 1.0-2.0% porcine serum, caseins were not hydrolysed by plasmin on incubation at 37 degrees C for 7 d. Porcine, and particularly equine, serum also impaired the coagulation of milk by chymosin or pepsin; equine serum (2.0%) increased the chymosin-induced coagulation time of milk similar to 4-fold and a firm chymosin-induced milk gel did not form. Adding heated (70 degrees C for 5 min) serum from any of the species to milk did not influence plasmin-induced hydrolysis of caseins or chymosin- or pepsin-induced coagulation of milk, suggesting that the inhibitor(s) is heat labile. Plasmin- or chymosin-induced hydrolysis of small synthetic Substrates was not influenced by blood serum. It is suggested that alpha(2)-macroglobulin may be the inhibitory substance, although further study is required to verify this. (c) 2006 Elsevier Ltd. All rights reserved.
Keyword Food Science & Technology
blood serum
milk
plasmin
chymosin
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
 
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Created: Fri, 25 Jan 2008, 16:50:14 EST