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Analysis of equid herpesvirus 1 strain variation reveals a point mutation of the DNA polymerase strongly associated with neuropathogenic versus nonneuropathogenic disease outbreaks
Nugent, J., Birch-Machin, I., Smith, K. C., Mumford, J. A., Swann, Z., Newton, J. R., Bowden, R. J., Allen, G. P. and Davis-Poynter, N. (2006-04) Analysis of equid herpesvirus 1 strain variation reveals a point mutation of the DNA polymerase strongly associated with neuropathogenic versus nonneuropathogenic disease outbreaks. Journal of Virology, 80 8: 4047-4060.
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| Author(s) |
Nugent, J.
Birch-Machin, I.
Smith, K. C.
Mumford, J. A.
Swann, Z.
Newton, J. R.
Bowden, R. J.
Allen, G. P.
Davis-Poynter, N.
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| Title |
Analysis of equid herpesvirus 1 strain variation reveals a point mutation of the DNA polymerase strongly associated with neuropathogenic versus nonneuropathogenic disease outbreaks
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| Journal name |
Journal of Virology
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| Publication date |
2006-04
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| Volume number |
80
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| Issue number |
8
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| ISSN |
0022-538X
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| Start page |
4047
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| End page |
4060
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| Total pages |
14
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| Place of publication |
Washington, D.C.
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| Publisher |
American Society for Microbiology
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| Language |
eng
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| Subject |
060506 Virology
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| Abstract |
Equid herpesvirus 1 (EHV-1) can cause a wide spectrum of diseases ranging from inapparent respiratory infection to the induction of abortion and, in extreme cases, neurological disease resulting in paralysis and ultimately death. It has been suggested that distinct strains of ERV-1 that differ in pathogenic capacity circulate in the field. In order to investigate this hypothesis, it was necessary to identify genetic markers that allow subgroups of related strains to be identified. We have determined all of the genetic differences between a neuropathogenic strain (Ab4) and a nonneuropathogenic strain (V592) of ERV-1 and developed PCR/sequencing procedures enabling differentiation of ERV-1 strains circulating in the field. The results indicate the occurrence of several major genetic subgroups of ERV-1 among isolates recovered from outbreaks over the course of 30 years, consistent with the proposal that distinct strains of EHV-1 circulate in the field. Moreover, there is evidence that certain strain groups are geographically restricted, being recovered predominantly from outbreaks occurring in either North America or Europe. Significantly, variation of a single amino acid of the DNA polymerase is strongly associated with neurological versus nonneurological disease outbreaks. Strikingly, this variant amino acid occurs at a highly conserved position for herpesvirus DNA polymerases, suggesting an important functional role.
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| Keyword(s) |
Virology
Varicella-zoster-virus
Human Cytomegalovirus
Glycoprotein-b
Molecular Epidemiology
Drug-resistance
Virological Characteristics
Rhinopneumonitis Vaccine
Interstrain Variation
Phylogenetic Analysis
Sequence Variation
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