Association of asthma with a functional promoter polymorphism in the IL16 gene

Burkart, Kristin M., Barton, Sheila J., Holloway, John W., Yang, Ian A., Cakebread, Julie A., Cruikshank, William, Little, Frederic, Jin, Xiaoyi, Farrier, Lindsay A., Clough, Joanne B., Keith, Tim P., Holgate, Stephen, Center, David M. and O'Connor, George T. (2006) Association of asthma with a functional promoter polymorphism in the IL16 gene. Journal of Allergy And Clinical Immunology, 117 1: 86-91. doi:10.1016/j.jaci.2005.10.011

Author Burkart, Kristin M.
Barton, Sheila J.
Holloway, John W.
Yang, Ian A.
Cakebread, Julie A.
Cruikshank, William
Little, Frederic
Jin, Xiaoyi
Farrier, Lindsay A.
Clough, Joanne B.
Keith, Tim P.
Holgate, Stephen
Center, David M.
O'Connor, George T.
Title Association of asthma with a functional promoter polymorphism in the IL16 gene
Journal name Journal of Allergy And Clinical Immunology   Check publisher's open access policy
ISSN 0091-6749
Publication date 2006-01
Sub-type Article (original research)
DOI 10.1016/j.jaci.2005.10.011
Volume 117
Issue 1
Start page 86
End page 91
Total pages 6
Editor D. Y. M. Leung
Place of publication St Louis
Publisher Mosby, Inc
Language eng
Subject C1
321027 Respiratory Diseases
730110 Respiratory system and diseases (incl. asthma)
Formatted abstract
Background: IL-16, a multifunctional cytokine with increased expression in the airways of asthmatic subjects, inhibits allergic airway inflammation in animal models. A T -> C single nucleotide polymorphism (SNP) at the -295 position in the promoter region of the IL16 gene has been described.

Objective: We sought to examine the functional significance of this promoter SNP and its relationship to asthma.

Methods: We examined the effect of the -295 SNP on promoter activity in cell-line (HBE4-E6/E7) transfection experiments. We investigated the association of the IL16 -295 genotype with asthma among 341 affected sib-pair white families and 184 unrelated nonasthmatic control subjects. We analyzed the association between the IL16 genotype and asthma using family-based association test and case-control analyses.

In in vitro transfection experiments the T allele in the -295 position was associated with substantially reduced promoter activity compared with the C allele. In the family study the more common T allele at the -295 position was significantly associated with all asthma phenotypes (P = .002 to P = .015). In the case-control analysis asthmatic subjects were more likely than unrelated nonasthmatic control subjects to have the -295 TT genotype, but this did not reach statistical significance (odds ratio, 1.36; 95% CI, 0.92-2.02).

The T allele at the -295 position in the IL16 promoter region is associated with reduced promoter activity relative to the C allele and with asthma in this white population. Further investigation is needed to delineate the mechanisms underlying these findings and the relationship of the IL16 -295 genotype to asthma in other populations.
Keyword Allergy
genetic epidemiology
family-based association test
single nucleotide polymorphism
Bronchoalveolar Lavage Fluid
Chemoattractant Activity
Caucasian Population
Interleukin 16
Il-16 Gene
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2007 Higher Education Research Data Collection
School of Medicine Publications
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Created: Fri, 25 Jan 2008, 16:46:52 EST