A new GLT1 splice variant: cloning and immunolocalization of GLT1c in the mammalian retina and brain

Rauen, T., Wiessner, M., Sullivan, R., Lee, A. and Pow, D. V. (2004) A new GLT1 splice variant: cloning and immunolocalization of GLT1c in the mammalian retina and brain. Neurochemistry International, 45 7: 1095-1106.


Author Rauen, T.
Wiessner, M.
Sullivan, R.
Lee, A.
Pow, D. V.
Title A new GLT1 splice variant: cloning and immunolocalization of GLT1c in the mammalian retina and brain
Journal name Neurochemistry International   Check publisher's open access policy
ISSN 0197-0186
Publication date 2004
Sub-type Article (original research)
DOI 10.1016/j.neuint.2004.04.006
Volume 45
Issue 7
Start page 1095
End page 1106
Total pages 12
Place of publication Oxford
Publisher Pergamon-Elsevier Science Ltd
Language eng
Abstract We have identified a novel carboxyl-terminal splice-variant of the glutamate transporter GLT1, which we denote as GLT1c. Within the rat brain only low levels of protein and message were detected, protein expression being restricted to end feet of astrocytes apposed to blood vessels or some astrocytes adjacent to the ventricles. Conversely, within the retina, this variant was selectively and heavily expressed in the synaptic terminals of both rod- and cone-photoreceptors in both humans and rats. Double-immunolabelling with antibodies to the carboxyl region of GLT1b/GLT1v, which is strongly expressed in apical dendrites of bipolar cells and in cone photoreceptors revealed that in the rat GLT1c was co-localised with GLT1b/GLT1v in cone photoreceptors but not with GLT1b/GLT1v in bipolar cells. GLT1c expression was developmentally regulated, only appearing at around postnatal day 7 in the rat retina, when photoreceptors first exhibit a dark current. Since the glutamate transporter EAAT5 is also expressed in terminals of rod photoreceptor terminals these data indicate that rod photoreceptors express two glutamate transporters with distinct properties. Similarly, cone photoreceptors express two glutamate transporters. We suggest that differential usage of these transporters by rod and cone photoreceptors may influence the kinetics of glutamate transmission by these neurons. (C) 2004 Elsevier Ltd. All rights reserved.
Keyword Biochemistry & Molecular Biology
Neurosciences
glutamate transporter
pre-synaptic
retina
splice variants
Glutamate Transporter Glt-1
Rat-brain
Tiger Salamander
Differential Expression
Localization
Neurons
Neurotransmitter
Photoreceptors
Homeostasis
Subtypes
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Biomedical Sciences Publications
 
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