The gene encoding the nucleocapsid protein of Gill-associated nidovirus of Penaeus monodon prawns is located upstream of the glycoprotein gene

Cowley, Jeff A., Cadogan, Lee C., Spann, Kirsten M., Sittidilokratna, Nusra and Walker, Peter J. (2004) The gene encoding the nucleocapsid protein of Gill-associated nidovirus of Penaeus monodon prawns is located upstream of the glycoprotein gene. Journal of Virology, 78 16: 8935-8941. doi:10.1128/JVI.78.16.8935-8941.2004

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
UQ123115_OA.pdf Full text (open access) application/pdf 1.00MB 0

Author Cowley, Jeff A.
Cadogan, Lee C.
Spann, Kirsten M.
Sittidilokratna, Nusra
Walker, Peter J.
Title The gene encoding the nucleocapsid protein of Gill-associated nidovirus of Penaeus monodon prawns is located upstream of the glycoprotein gene
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
1098-5514
Publication date 2004-08-01
Year available 2004
Sub-type Article (original research)
DOI 10.1128/JVI.78.16.8935-8941.2004
Open Access Status File (Publisher version)
Volume 78
Issue 16
Start page 8935
End page 8941
Total pages 7
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract The ORF2 gene of Gill-associated virus (GAV) of Penaeus monodon prawns resides 93 nucleotides downstream of the ORF1a-ORF1b gene and encodes a 144-amino-acid hydrophilic polypeptide (15,998 Da; pI, 9.75) containing 20 basic (14%) and 13 acidic (9%) residues and 19 prolines (13%). Antiserum to a synthetic ORF2 peptide or an Escherichia coli-expressed glutathione S-transferase-ORF2 fusion protein detected a 20-kDa protein in infected lymphoid organ and gill tissues in Western blots. The GAV ORF2 fusion protein antiserum also cross-reacted with the p20 nucleoprotein in virions of the closely related Yellow head virus. By immuno-gold electron microscopy, it was observed that the ORF2 peptide antibody localized to tubular GAV nucleocapsids, often at the ends or at lateral cross sections. As GAV appears to contain only two structural protein genes (ORF2 and ORF3), these data indicate that GAV differs from vertebrate nidoviruses in that the gene encoding the nucleocapsid protein is located upstream of the gene encoding the virion glycoproteins.
Keyword Virology
Yellow-head Virus
Dehydrogenase-elevating Virus
In-situ Hybridization
Lymphoid Organ Virus
Berne Virus
Invertebrate Nidovirus
Rna-transcription
Causative Agent
Messenger-rnas
Sequence
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Clinical Medical Virology Centre Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 19 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 17 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 25 Jan 2008, 16:18:54 EST