A pre-ribosome with a short article tadpole-like structure functions in ATP-dependent maturation of 60S subunits

Nissan, Tracy A., Galani, Kyriaki, Maco, Bohumil, Tollervey, David, Aebi, Ueli and Hurt, Ed (2004) A pre-ribosome with a short article tadpole-like structure functions in ATP-dependent maturation of 60S subunits. Molecular Cell, 15 2: 295-301.


Author Nissan, Tracy A.
Galani, Kyriaki
Maco, Bohumil
Tollervey, David
Aebi, Ueli
Hurt, Ed
Title A pre-ribosome with a short article tadpole-like structure functions in ATP-dependent maturation of 60S subunits
Journal name Molecular Cell  (ERA 2012 Listed)    (ERA 2010 Rank A*)   Check publisher's open access policy
Publication date 2004-07
Sub-type Article
DOI 10.1016/j.molcel.2004.06.033
Volume number 15
Issue number 2
ISSN 1097-2765
Start page 295
End page 301
Total pages 7
Place of publication Cambridge, Mass. U.S.A.
Publisher Cell Press
Language eng
Subject 100401 Gene and Molecular Therapy
Abstract Analyses of isolated pre-ribosomes yielded biochemical "snapshots" of the dynamic, nascent 60S and 40S subunits during their path from the nucleolus to the cytoplasm. Here, we present the structure of a pre-60S ribosomal intermediate located in the nucleoplasm. A huge dynein-related AAA-type ATPase (Real) and the Rix1 complex (Rix1-lpi1-lpi3) are components of an extended (similar to45 nm long) pre-60S particle. Antibody crosslinking in combination with electron microscopy revealed that the Real localizes to the "tail" region and ribosomal proteins to the "head" region of the elongated "tadpole-like" structure. Furthermore, in vitro treatment with ATP induces dissociation of Real from the pre-60S subunits. Real and the Rix1 complex could mediate ATP-dependent remodeling of 60S subunits and subsequent export from the nucleoplasm to the cytoplasm.
Keyword Biochemistry & Molecular Biology
Cell Biology
Saccharomyces-cerevisiae
Protein
Rna
Export
Biogenesis
Nucleolus
Cytoplasm
Particle
Dynein
Gtpase
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article
Collections: Excellence in Research Australia (ERA) - Collection
School of Chemistry and Molecular Biosciences
 
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